Helpap B, Grouls V, Hesse U
Cryobiology. 1985 Apr;22(2):168-74. doi: 10.1016/0011-2240(85)90171-3.
Focal thermo- and cryolesions were placed on one kidney and the liver of Wistar rats. The wound healing was investigated histologically and by tritiated thymidine autoradiography. Furthermore, the 3H labeling indices of neutrophilic and eosinophilic promyelocytes and myelocytes as well as that of erythroblasts were determined in bone marrow samples from femur and vertebra. Normal nonoperated rats and rats which underwent sham operation (simple laparotomy) served as controls. The cryonecrosis healed completely after 4 weeks, whereas thermonecrotic areas were still observed 12 weeks after operation. The maximum mesenchymal and epithelial proliferation occurred between the 2nd and 3rd postoperative days. In the bone marrow, the highest labeling indices were estimated in animals with thermonecrosis, with values remaining increased during the 2 weeks after operation. Significant differences between the bone marrow response after cryolesions and sham operation were not observed. Thermolesions of internal organs, for example, after electrocoagulation, evoke a persisting local granulomatous inflammation accompanied by a distinct bone marrow reaction probably because of the pure resorption of carbonized tissue components. Iatrogenic cryolesions, in contrast, are characterized by a quick and uncomplicated wound healing without a significantly increased proliferation of bone marrow cells, thus corresponding to the wound healing after sham operation.
在Wistar大鼠的一侧肾脏和肝脏上进行局灶性热损伤和冷冻损伤。通过组织学检查和氚标记胸腺嘧啶核苷放射自显影术研究伤口愈合情况。此外，还测定了来自股骨和椎骨的骨髓样本中嗜中性和嗜酸性早幼粒细胞、中幼粒细胞以及成红细胞的3H标记指数。正常未手术的大鼠和接受假手术（单纯剖腹术）的大鼠作为对照。冷冻坏死在4周后完全愈合，而热坏死区域在术后12周仍可观察到。最大的间充质和上皮细胞增殖发生在术后第2天和第3天之间。在骨髓中，热坏死动物的标记指数最高，术后2周内其值持续升高。未观察到冷冻损伤后骨髓反应与假手术之间的显著差异。例如，电凝后内脏的热损伤会引发持续的局部肉芽肿性炎症，并伴有明显的骨髓反应，这可能是由于碳化组织成分的单纯吸收所致。相比之下，医源性冷冻损伤的特点是伤口愈合迅速且无并发症，骨髓细胞增殖未显著增加，因此与假手术后的伤口愈合情况相符。
