Helpap B, Grouls V, Lange O, Breining H, Lymberopoulos S
Pathol Res Pract. 1979 May;164(2):167-77. doi: 10.1016/S0344-0338(79)80021-7.
In order to evaluate a possible primary or secondary immunologic response of the spleen after single or repeated in situ freezing of parenchymal organs such as liver and kidney within a four week period, light microscopic and cell kinetic investigations with tritiated thymidine were performed on spleens of non-germfree rats. Sham operations served as controls. The sham operations did not induce any significant morphological or cell kinetic changes in the splenic white pulp. After cryolesions were produced in the liver and kidney, the percentages of activated germinal centers, labeled germinal center cells, and cells in the perifollicular area of the lymphatic mantle and marginal zones increased, with maxima during the first 3 days. The investigations show that the cellular reaction of the spleen starts earlier and is more prominent after repeated in situ freezing than after a single cryolesion. These findings point to an immunologic response of the anamnestic type, and correspond to results after repeated freezing of normal and malignant tissue of the urogenital tract. These cell kinetic results are important in the evaluation of further immunologic studies involving the cryotherapy of malignant tissues.
为了评估在四周内对肝脏和肾脏等实质器官进行单次或重复原位冷冻后脾脏可能产生的原发性或继发性免疫反应，对非无菌大鼠的脾脏进行了光学显微镜检查和用氚标记胸腺嘧啶核苷的细胞动力学研究。假手术作为对照。假手术未在脾脏白髓中引起任何显著的形态学或细胞动力学变化。在肝脏和肾脏产生冷冻损伤后，活化生发中心、标记生发中心细胞以及淋巴套和边缘区滤泡周围区域细胞的百分比增加，在最初3天达到最大值。研究表明，与单次冷冻损伤相比，重复原位冷冻后脾脏的细胞反应开始得更早且更显著。这些发现表明存在回忆型免疫反应，并且与泌尿生殖道正常组织和恶性组织重复冷冻后的结果一致。这些细胞动力学结果对于评估涉及恶性组织冷冻治疗的进一步免疫学研究具有重要意义。
