Yang Yang, Yang Wei, Shen Jie, Ding Enci
The First Central Clinical School, Tianjin Medical University, Tianjin 300000, P.R. China.
Department of Cardiology, Fuwai Central China Cardiovascular Hospital, Zhengzhou, Henan 450000, P.R. China.
Mol Med Rep. 2025 Mar;31(3). doi: 10.3892/mmr.2025.13431. Epub 2025 Jan 10.
Hepatocellular carcinoma (HCC) is a common cause of cancer‑related mortality and morbidity worldwide. While iodine‑125 (I) particle brachytherapy has been extensively used in the clinical treatment of various types of cancer, the precise mechanism underlying its effectiveness in treating HCC remains unclear. In the present study, MHCC‑97H cells were treated with I, after which, cell viability and proliferation were assessed using Cell Counting Kit‑8, 5‑ethynyl‑2'‑deoxyuridine and colony formation assays, cell invasion and migration were evaluated using wound healing and Transwell assays, and cell apoptosis was determined using flow cytometry. Omics data were analyzed using Kyoto Encyclopedia of Genes and Genomes, Gene Ontology and STRING analyses to observe the key genes that exhibited significant changes at the transcriptional and protein levels in MHCC‑97H cells treated with I particles. Finally, the expression levels of key genes (GPNMB, C4BPA, CTH, H1‑0 and MT2A) were verified through reverse transcription quantitative PCR. Following treatment with I, the proliferation, invasion and migration of MHCC‑97H cells were inhibited, and apoptosis was enhanced. The results of omics data analysis indicated that the biological behavior of MHCC‑97H cells treated with I was related to the expression levels of CTH and MT2A genes. These findings indicated that intervention with I radiation particles may induce changes in gene expression, potentially influencing alterations in biological characteristics. In conclusion, these insights may shed light on the underlying mechanisms of I radiation particle therapy in HCC and offer novel targets for HCC treatment.
肝细胞癌（HCC）是全球癌症相关死亡率和发病率的常见原因。虽然碘-125（I）粒子近距离放射治疗已广泛应用于各种类型癌症的临床治疗，但其治疗HCC有效性的精确机制仍不清楚。在本研究中，用I处理MHCC-97H细胞，之后，使用细胞计数试剂盒-8、5-乙炔基-2'-脱氧尿苷和集落形成试验评估细胞活力和增殖，使用伤口愈合试验和Transwell试验评估细胞侵袭和迁移，并使用流式细胞术测定细胞凋亡。使用京都基因与基因组百科全书、基因本体论和STRING分析对组学数据进行分析，以观察在用I粒子处理的MHCC-97H细胞中在转录和蛋白质水平上表现出显著变化的关键基因。最后，通过逆转录定量PCR验证关键基因（GPNMB、C4BPA、CTH、H1-0和MT2A）的表达水平。用I处理后，MHCC-97H细胞的增殖、侵袭和迁移受到抑制，凋亡增强。组学数据分析结果表明，用I处理的MHCC-97H细胞的生物学行为与CTH和MT2A基因的表达水平有关。这些发现表明，I辐射粒子干预可能诱导基因表达变化，潜在地影响生物学特性的改变。总之，这些见解可能有助于揭示I辐射粒子治疗HCC的潜在机制，并为HCC治疗提供新的靶点。
