Gray Holly A, Biggs Patrick J, Midwinter Anne C, Rogers Lynn E, Fayaz Ahmed, Akhter Rukhshana N, Burgess Sara A
mEpiLab, School of Veterinary Science, Massey University, Palmerston North, New Zealand.
School of Food Technology and Natural Sciences, Massey University, Palmerston North, New Zealand.
Microb Genom. 2025 Jan;11(1). doi: 10.1099/mgen.0.001341.
In Aotearoa New Zealand, urinary tract infections in humans are commonly caused by extended-spectrum beta-lactamase (ESBL)-producing . This group of antimicrobial-resistant bacteria are often multidrug resistant. However, there is limited information on ESBL-producing found in the environment and their link with human clinical isolates. In this study, we examined the genetic relationship between environmental and human clinical ESBL-producing and isolates collected in parallel within the same area over 14 months. Environmental samples were collected from treated effluent, stormwater and multiple locations along an Aotearoa New Zealand river. Treated effluent, stormwater and river water sourced downstream of the treated effluent outlet were the main samples that were positive for ESBL-producing (7/14 samples, 50.0%; 3/6 samples, 50%; and 15/28 samples, 54%, respectively). Whole-genome sequence comparison was carried out on 307 human clinical and 45 environmental ESBL-producing isolates. Sequence type 131 was dominant for both clinical (147/307, 47.9%) and environmental isolates (11/45, 24.4%). Only one ESBL gene was detected in each isolate. Among the clinical isolates, the most prevalent ESBL genes were (134/307, 43.6%) and (134/307, 43.6%). Among the environmental isolates, (28/45, 62.2%) was the most prevalent gene. A core SNP analysis of these isolates suggested that some strains were shared between humans and the local river. These results highlight the importance of understanding different transmission pathways for the spread of ESBL-producing .
在新西兰奥特亚罗瓦，人类尿路感染通常由产超广谱β-内酰胺酶（ESBL）的[细菌名称未给出]引起。这组耐抗菌药物细菌往往具有多重耐药性。然而，关于环境中发现的产ESBL的[细菌名称未给出]及其与人类临床分离株的联系的信息有限。在本研究中，我们检测了在14个月内于同一地区并行收集的环境和人类临床产ESBL的[细菌名称未给出]及分离株之间的遗传关系。环境样本取自处理后的废水、雨水以及新西兰奥特亚罗瓦一条河流沿线的多个地点。处理后的废水、雨水以及处理后废水排放口下游的河水是产ESBL的[细菌名称未给出]呈阳性的主要样本（分别为7/14个样本，50.0%；3/6个样本，50%；以及15/28个样本，54%）。对307株人类临床产ESBL的分离株和45株环境产ESBL的分离株进行了全基因组序列比较。序列类型131在临床分离株（147/307，47.9%）和环境分离株（11/45，24.4%）中均占主导。每个分离株仅检测到一个ESBL基因。在临床分离株中，最常见的ESBL基因是[基因名称未给出]（134/307，43.6%）和[基因名称未给出]（134/307，43.6%）。在环境分离株中，[基因名称未给出]（28/45，62.2%）是最常见的基因。对这些分离株的核心单核苷酸多态性分析表明，人类和当地河流之间存在一些共享菌株。这些结果凸显了了解产ESBL的[细菌名称未给出]传播的不同途径的重要性。
