Wang Jingjing, Ye Fang, Chai Haoxi, Jiang Yujia, Wang Teng, Ran Xia, Xia Qimin, Xu Ziye, Fu Yuting, Zhang Guodong, Wu Hanyu, Guo Guoji, Guo Hongshan, Ruan Yijun, Wang Yongcheng, Xing Dong, Xu Xun, Zhang Zemin
Bone Marrow Transplantation Center of the First Affiliated Hospital & Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou, 310058, China.
Life Sciences Institute and The Second Affiliated Hospital, Zhejiang University, Hangzhou, 310058, China.
Sci China Life Sci. 2024 Dec 20. doi: 10.1007/s11427-024-2770-x.
The applications of single-cell and spatial technologies in recent times have revolutionized the present understanding of cellular states and the cellular heterogeneity inherent in complex biological systems. These advancements offer unprecedented resolution in the examination of the functional genomics of individual cells and their spatial context within tissues. In this review, we have comprehensively discussed the historical development and recent progress in the field of single-cell and spatial genomics. We have reviewed the breakthroughs in single-cell multi-omics technologies, spatial genomics methods, and the computational strategies employed toward the analyses of single-cell atlas data. Furthermore, we have highlighted the advances made in constructing cellular atlases and their clinical applications, particularly in the context of disease. Finally, we have discussed the emerging trends, challenges, and opportunities in this rapidly evolving field.
近年来，单细胞和空间技术的应用彻底改变了我们目前对细胞状态以及复杂生物系统中固有细胞异质性的理解。这些进展为检查单个细胞的功能基因组学及其在组织中的空间背景提供了前所未有的分辨率。在这篇综述中，我们全面讨论了单细胞和空间基因组学领域的历史发展和最新进展。我们回顾了单细胞多组学技术、空间基因组学方法以及用于分析单细胞图谱数据的计算策略方面的突破。此外，我们强调了在构建细胞图谱及其临床应用方面取得的进展，特别是在疾病背景下。最后，我们讨论了这个快速发展领域中的新兴趋势、挑战和机遇。
