Krystev L P, Angelova P A
Eksp Onkol. 1985;7(1):24-6.
The morphology and proliferative activity of liver cells from chick embryos were studied. In vitro, the cells were treated with N-nitrosodiethylamine (NDEA) in doses of 100 and 75 mg/ml of culture medium for a 10-day period. The primary culture from liver cells of 9-day chick embryos was prepared on the highly permeable celloidine membrane. In early periods of cultivation there were degenerative signs, mainly in the parenchymal cells, which shows a cytotoxic effect of NDEA. Proliferation of a small population of light epithelioid cells was observed towards the end of the experiment. There were also some atypical and pathological mitoses in the fibroblasts. The obtained results show that a neoplastic transformation of the chick embryonal liver cells occurs under in vitro conditions.
研究了鸡胚肝细胞的形态和增殖活性。在体外，将细胞用剂量为100和75毫克/毫升培养基的N-亚硝基二乙胺（NDEA）处理10天。在高渗透性火棉胶膜上制备了9日龄鸡胚肝细胞的原代培养物。在培养早期出现了退化迹象，主要在实质细胞中，这显示了NDEA的细胞毒性作用。在实验结束时观察到一小群轻度上皮样细胞的增殖。成纤维细胞中也有一些非典型和病理性有丝分裂。所得结果表明，鸡胚肝细胞在体外条件下会发生肿瘤转化。
