Gouda Mohamed, Khalaf Mai M, Abou Taleb Manal F, Alali Ibtisam, Abd El-Lateef Hany M
Department of Chemistry, College of Science, King Faisal University, Al-Ahsa 31982, Saudi Arabia.
Department of Chemistry, College of Science, King Faisal University, Al-Ahsa 31982, Saudi Arabia.
Int J Biol Macromol. 2025 Mar;296:139658. doi: 10.1016/j.ijbiomac.2025.139658. Epub 2025 Jan 8.
In this work, chitosan (Cs) was blended with different concentrations of Origanum majorana extract (OmE) that extracted using ethyl acetate and used for the formation of food packaging films. Based on the utilized volumes of OmE (2.5, 5, and 7.5 mL) that were mixed with 27.5, 25, and 22.5 mL of Cs, these different film samples (2.5 OmE loaded Cs film, 5 OmE loaded Cs film and 7.5 OmE loaded Cs film) were obtained and compared with the film of pure Cs. The extraction yields of OmE were found to be 13 g and 10.4 g % when using ethyl acetate (OmE) and methanol (OmME), respectively, with total phenolic content measured at 889.30 μg GAE/g for the ethyl acetate extract and 810.21 μg GAE/g for the methanol extract, indicating a substantial amount of bioactive compounds available for formulation. Antimicrobial activity was assessed against various foodborne pathogens, with the 7.5 OmE loaded Cs film demonstrating the highest efficacy, achieving inhibition zones of 27 mm against E. coli and 25 mm against S. aureus. This research underscores the potential of Cs-based films loaded with O. majorana extract as a viable solution for active food packaging, addressing environmental concerns and food safety.
在这项工作中，壳聚糖（Cs）与不同浓度的用乙酸乙酯提取的马郁兰提取物（OmE）混合，并用于制备食品包装薄膜。根据与27.5、25和22.5 mL的Cs混合的OmE的使用量（2.5、5和7.5 mL），获得了这些不同的薄膜样品（2.5 OmE负载的Cs薄膜、5 OmE负载的Cs薄膜和7.5 OmE负载的Cs薄膜），并与纯Cs薄膜进行了比较。发现使用乙酸乙酯（OmE）和甲醇（OmME）时，OmE的提取率分别为13 g和10.4 g%，乙酸乙酯提取物的总酚含量为889.30 μg GAE/g，甲醇提取物的总酚含量为810.21 μg GAE/g，表明有大量生物活性化合物可用于配方。评估了对各种食源性病原体的抗菌活性，7.5 OmE负载的Cs薄膜显示出最高的功效，对大肠杆菌的抑菌圈为27 mm，对金黄色葡萄球菌的抑菌圈为25 mm。这项研究强调了负载马郁兰提取物的基于Cs的薄膜作为活性食品包装的可行解决方案的潜力，解决了环境问题和食品安全问题。
