Righi Halima, Bekhti-Sari Fadia, Cherrak Sabri Ahmed, Bettioui Reda, Dahmani Dahbia Ines, Ayachi Ouarda Sariyah, Bensenane Bachir, Belloufa Ahmed, Merzouk Hafida, Mokhtari-Soulimane Nassima Amal
Laboratory of Physiology, Pathophysiology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University of Tlemcen, Tlemcen 13000, Algeria.
Laboratory of Physiology, Pathophysiology and Biochemistry of Nutrition, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, University of Tlemcen, Tlemcen 13000, Algeria.
Int J Biol Macromol. 2025 Mar;295:139693. doi: 10.1016/j.ijbiomac.2025.139693. Epub 2025 Jan 8.
Camel α-Lactalbumin (α-LAC) has been shown to exert bioactivities for Reactive oxygen species (ROS) scavenging and anti-inflammation, showing the ability to treat obesity-related metabolic disorders. Herein, we present a novel process to purify α-LAC in a single chromatographic step from camel whey in a flow-through format. We also demonstrate the role of α-LAC modulation strategies for the treatment of obesity. An in-vivo study was systematically carried-out, in which an intragastric administration of α-LAC in rats experiencing obesity showed ameliorating effect on hepatic steatosis and dyslipidemia. In addition, we demonstrated that the reduction of inflammation and oxidative stress by α-LAC was effective, and importantly, the enhancement of antioxidant defenses was achieved. Furthermore, we found that the peroxynitrite scavenging ability of α-LAC could help restore the hepatorenal function. Finally, we showed that α-LAC could improve insulin resistance-associated glucose metabolism and insulin sensitivity disorders caused by obesity. This work has developed a novel one-step purification process of α-LAC from camel whey without the use of organic solvents. Our purified α-LAC may serve as a promising therapeutic agent in the treatment of obesity-related metabolic disorders by synergistically alleviating lipid accumulation, inflammation, oxidative stress and insulin sensitivity.
骆驼α-乳白蛋白（α-LAC）已被证明具有清除活性氧（ROS）和抗炎的生物活性，显示出治疗肥胖相关代谢紊乱的能力。在此，我们提出了一种新颖的方法，以流通形式通过单一色谱步骤从骆驼乳清中纯化α-LAC。我们还证明了α-LAC调节策略在治疗肥胖中的作用。系统地进行了一项体内研究，其中对肥胖大鼠进行胃内给予α-LAC对肝脂肪变性和血脂异常显示出改善作用。此外，我们证明α-LAC减轻炎症和氧化应激是有效的，重要的是，实现了抗氧化防御的增强。此外，我们发现α-LAC清除过氧亚硝酸盐的能力有助于恢复肝肾功。最后，我们表明α-LAC可以改善由肥胖引起的胰岛素抵抗相关的葡萄糖代谢和胰岛素敏感性障碍。这项工作开发了一种新颖的从骆驼乳清中一步纯化α-LAC的方法，无需使用有机溶剂。我们纯化的α-LAC通过协同减轻脂质积累、炎症、氧化应激和胰岛素敏感性，可能成为治疗肥胖相关代谢紊乱的有前景的治疗剂。
