Nguyen Hoang-Duy P, Le Bao-Ngoc T, Nguyen Hong-Nhung, Nguyen Thuy-Van T, Duong Thanh-Linh H, Hoang Tien-Cuong, Duy Nguyen Phuc Thanh, Nguyen Minh V, Duong Linh N, Le Loan Q, Pham Thuy-Phuong T
Institute of Chemical Technology - Vietnam Academy of Science and Technology, 1A TL29 Street, Thanh Loc Ward, District 12, Ho Chi Minh City, Vietnam.
Faculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT), 268 Ly Thuong Kiet St., District 10, Ho Chi Minh City, Vietnam.
World J Microbiol Biotechnol. 2025 Jan 11;41(2):31. doi: 10.1007/s11274-025-04249-2.
This study demonstrated a novel approach to accurately estimate 5-day biochemical oxygen demand (BOD) in textile wastewater using a microbial consortium from food processing wastewater fixed on coconut fibers. Although glucose-glutamic acid (GGA) has been widely known as the most preferred substrates for microbial respiration, its calibration surprisingly resulted in an overestimation of BOD in textile wastewater due to its lower utilization rate compared to that of textile wastewater. After being adapted with a new nutrient environment composed of GGA and textile wastewater, the adapted packed-bed bioreactors (PBBRs) was capable of accurate estimation of BOD in textile wastewater using GGA standard solution. Metagenomic analysis revealed the dominance of the genera Enterobacter, Acinetobacter, Chryseobacterium, and Comamonas in the adapted microbial community, which are recognized for their significant potential in azo dye degradation. The imputed metagenome showed an enhanced showed an enhanced abundance of "Amino Acid Degradation" and "Carbohydrate Degradation" functions, confirming the improved ability of adapted community to utilization of GGA in the standard solution. These findings suggest that adaptation of exogenous microbial consortium to a nutrient environment composed of GGA and target wastewater may shift the community to that dominated by strains having both utilization ability of GGA and target compounds which, in turn, enhance the accuracy of the adapted PBBRs for estimation of BOD in target wastewater.
本研究展示了一种新方法，即使用固定在椰壳纤维上的食品加工废水微生物群落准确估算纺织废水中的五日生化需氧量（BOD）。尽管葡萄糖 - 谷氨酸（GGA）作为微生物呼吸最优选的底物已广为人知，但其校准却出人意料地导致纺织废水中BOD的高估，因为与纺织废水相比其利用率较低。在用由GGA和纺织废水组成的新营养环境进行驯化后，经驯化的填充床生物反应器（PBBRs）能够使用GGA标准溶液准确估算纺织废水中的BOD。宏基因组分析揭示了肠杆菌属、不动杆菌属、金黄杆菌属和丛毛单胞菌属在驯化微生物群落中的优势地位，这些属因其在偶氮染料降解方面的巨大潜力而闻名。推算的宏基因组显示“氨基酸降解”和“碳水化合物降解”功能的丰度增加，证实了驯化群落利用标准溶液中GGA能力的提高。这些发现表明，使外源微生物群落适应由GGA和目标废水组成的营养环境可能会使群落转变为由具有GGA和目标化合物利用能力的菌株主导，进而提高经驯化的PBBRs估算目标废水中BOD的准确性。
