School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, 529020, China.
School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, 529020, China.
Talanta. 2023 Aug 15;261:124671. doi: 10.1016/j.talanta.2023.124671. Epub 2023 May 13.
Biochemical oxygen demand (BOD) is a water quality parameter of vital importance. Rapid BOD analysis methods have emerged to simplify the five-day BOD (BOD) measurement protocol. However, their universal implementations are restricted by the tricky environmental matrix (including environmental microbes, contaminants, ionic compositions, etc.). Here, an in situ and self-adaptive BOD bioreaction sensing system consisting of a "gut-like" microfluidic coil bioreactor with self-renewed biofilm was proposed for the establishment of a rapid, resilient and reliable BOD determination method. With the spontaneous surface adhesion of environmental microbial populations, the biofilm was colonized in situ on the inner surface of the microfluidic coil bioreactor. Exploiting the environmental domestication during every real sample measurement, the biofilm was capable of self-renewal to adapt to the environmental changes and exhibited representative biodegradation behaviors. The aggregated abundant, adequate and adapted microbial populations in the BOD bioreactor rendered a total organic carbon (TOC) removal rate of 67.7% within a short hydraulic retention time of 99 s. As validated by an online BOD prototype, exceptional analytical performance was achieved in terms of reproducibility (relative standard deviation of 3.7%), survivability (inhibition by pH and metal ion interference of <20%) and accuracy (relative error of -5.9% to 9.7%). This work rediscovered the interactive effects of the environmental matrix on BOD assays and demonstrated an instructive attempt by making use of the environment to develop practical online BOD monitoring devices for water quality assessments.
生化需氧量（BOD）是一项非常重要的水质参数。为了简化五日生化需氧量（BOD）的测量方法，已经出现了快速 BOD 分析方法。然而，由于环境基质（包括环境微生物、污染物、离子组成等）的复杂性，这些方法的普遍应用受到了限制。在这里，我们提出了一种原位自适应性 BOD 生物反应传感系统，该系统由一个具有自更新生物膜的“肠道样”微流控螺旋生物反应器组成，用于建立一种快速、有弹性和可靠的 BOD 测定方法。通过环境微生物种群的自发表面附着，生物膜原位定殖在微流控螺旋生物反应器的内表面上。利用每次实际样品测量中的环境驯化，生物膜能够自我更新以适应环境变化，并表现出有代表性的生物降解行为。在 BOD 生物反应器中，聚集的丰富、充足和适应性强的微生物种群在 99 秒的短水力停留时间内实现了 67.7%的总有机碳（TOC）去除率。通过在线 BOD 原型验证，该方法在重现性（相对标准偏差为 3.7%）、生存能力（pH 和金属离子干扰的抑制率<20%）和准确性（相对误差为-5.9%至 9.7%）方面都取得了优异的分析性能。这项工作重新发现了环境基质对 BOD 测定的相互作用，并通过利用环境来开发实用的在线 BOD 监测设备以评估水质，展示了一个有益的尝试。
