Department of Food and Nutrition, University of Helsinki, Helsinki 00014, Finland.
Key Laboratory of Geriatric Nutrition and Health, Beijing Technology and Business University, Ministry of Education, Beijing 100048, China.
Poult Sci. 2024 Aug;103(8):103877. doi: 10.1016/j.psj.2024.103877. Epub 2024 May 17.
This study aims to provide new insight on the association between the development of wooden breast myopathy and mitochondrial and glycolytic activity under oxidative stress. Myopathic muscle had higher oxidative stress together with altered glycolytic metabolism and tricarboxylic acid (TCA) cycle. This was evidenced by significantly elevated antioxidant enzyme activities (catalase, superoxide dismutase, and glutathione peroxidase), decreased citrate synthase activity and postmortem glycolytic potential with increasing wooden breast severity. In addition, affected muscles also exhibited higher initial and ultimate pH values as well as reduced total glucose and lactate contents. Citrate synthase activity was negatively correlated to antioxidant enzyme activities. Taken together, we propose that the development of the wooden breast lesion is a chronic process that may be related to the failure of muscle fibers to defend against the excessively generated oxidative products promoted by mitochondrial damage accompanied by impaired TCA cycle. Furthermore, there was a positive correlation between citrate synthase activity and glycolytic potential, which suggests that the wooden breast condition is linked to the overall altered energy metabolism of the muscle, including the oxidative phosphorylation and glycolytic pathways.
本研究旨在探讨氧化应激下木质化鸡胸肉肌病的发生与线粒体和糖酵解活性之间的关系。病态肌肉的氧化应激水平较高，同时伴有糖酵解代谢和三羧酸（TCA）循环改变。这一点可以从抗氧化酶活性（过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶）显著升高、柠檬酸合酶活性降低以及木质化鸡胸肉严重程度增加导致的死后糖酵解潜力下降得到证明。此外，受影响的肌肉还表现出更高的初始和最终 pH 值以及总葡萄糖和乳酸含量降低。柠檬酸合酶活性与抗氧化酶活性呈负相关。综上所述，我们提出木质化鸡胸肉病变的发生是一个慢性过程，可能与肌肉纤维对线粒体损伤引起的过度生成的氧化产物的防御失败有关，同时伴有 TCA 循环受损。此外，柠檬酸合酶活性与糖酵解潜力呈正相关，这表明木质化鸡胸肉的发生与肌肉整体改变的能量代谢有关，包括氧化磷酸化和糖酵解途径。
