He Fan, Li Mingguo, Chen Fan, Zhou Rong, Qi Mengfan, Fu Binbin, Zhang Huapeng, Li Qingchun, Bi Yanzhen, Huang Tao
College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.
Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.
Animals (Basel). 2024 Dec 27;15(1):46. doi: 10.3390/ani15010046.
Early embryonic development relies on intricately regulated gene expression, and miRNAs influence zygotic genome activation (ZGA), cleavage, and cell fate determination through post-transcriptional regulatory mechanisms. miR-192 is expressed in early pig embryos and participates in various reproductive processes. However, its role in pre-implantation pig embryo development remains poorly understood. In this study, we microinjected the miR-192 agonist (miR-192 agomir) into parthenogenetically activated pig embryos to evaluate its effects on early pig embryo development. Our findings indicate that compared to the control group (agomir NC), miR-192 agomir impairs the developmental capacity of parthenogenetic pig embryos to reach the 2-cell, 4-cell, and blastocyst stages. This impairment leads to imbalances in the oxidative-reductive system and abnormalities in mitochondrial function during the 4-cell stage, resulting in the significant accumulation of ROS, notable decreases in the expression of antioxidant enzymes CAT and SOD1 mRNA, reduction in mitochondrial membrane potential, and induction of apoptosis in pig blastocysts. Additionally, the overexpression of miR-192 inhibits the expression of its target genes YY1 and the pluripotency factor NANOG mRNA. In conclusion, this study reveals that the overexpression of miR-192 adversely affects early pig embryo development, providing new evidence for understanding the role miR-192 plays in reproduction.
早期胚胎发育依赖于精确调控的基因表达，而微小RNA（miRNA）通过转录后调控机制影响合子基因组激活（ZGA）、卵裂和细胞命运决定。miR-192在猪早期胚胎中表达，并参与各种生殖过程。然而，其在猪植入前胚胎发育中的作用仍知之甚少。在本研究中，我们将miR-192激动剂（miR-192 agomir）显微注射到孤雌激活的猪胚胎中，以评估其对猪早期胚胎发育的影响。我们的研究结果表明，与对照组（agomir NC）相比，miR-192 agomir损害了孤雌猪胚胎发育至2细胞、4细胞和囊胚阶段的能力。这种损害导致4细胞阶段氧化还原系统失衡和线粒体功能异常，导致活性氧（ROS）大量积累、抗氧化酶CAT和SOD1 mRNA表达显著降低、线粒体膜电位降低以及猪囊胚细胞凋亡。此外，miR-192的过表达抑制了其靶基因YY1和多能性因子NANOG mRNA的表达。总之，本研究揭示了miR-192的过表达对猪早期胚胎发育产生不利影响，为理解miR-192在生殖中的作用提供了新的证据。
