Ridlo Muhammad Rosyid, Kim Eui Hyun, Kim Geon A
Department of Theriogenology and Biotechnology, Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
Department of Bioresources Technology and Veterinary, Vocational College, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Animals (Basel). 2021 Jan 18;11(1):221. doi: 10.3390/ani11010221.
Endoplasmic reticulum (ER) stress can be triggered during in vitro embryo production and is a major obstacle to embryo survival. MicroRNA (miR)-210 is associated with cellular adaptation to cellular stress and inflammation. An experiment was conducted to understand the effects of miR-210 on in vitro embryo development, ER stress, and apoptosis; to achieve this, miR-210 was microinjected into parthenogenetically activated embryos. Our results revealed that miR-210 inhibition significantly enhanced the cleavage rate, blastocyst formation rate, and total cell number (TCN) of blastocysts, and reduced expression levels of ( < 0.05). miR-210 inhibition greatly reduced the expression of ER stress-related genes (, , , and ) and and increased the levels of and ( < 0.05). A miR-210-mimic significantly decreased the cleavage, blastocyst rate, TCN, and expression levels of compared with other groups ( < 0.05). The miR-210-mimic impaired the expression levels of , , , , and and decreased the expression of and ( < 0.05). In conclusion, miR-210 plays an essential role in porcine in vitro embryo development. Therefore, we suggest that miR-210 inhibition could alleviate ER stress and reduce apoptosis to support the enhancement of in vitro embryo production.
内质网（ER）应激可在体外胚胎生产过程中被触发，是胚胎存活的主要障碍。微小RNA（miR）-210与细胞对细胞应激和炎症的适应性相关。进行了一项实验以了解miR-210对体外胚胎发育、内质网应激和细胞凋亡的影响；为此，将miR-210显微注射到孤雌激活的胚胎中。我们的结果显示，抑制miR-210可显著提高囊胚的分裂率、囊胚形成率和总细胞数（TCN），并降低（<0.05）的表达水平。抑制miR-210可大大降低内质网应激相关基因（、、、和）以及的表达，并提高和的水平（<0.05）。与其他组相比，miR-210模拟物显著降低了分裂率、囊胚率、TCN以及的表达水平（<0.05）。miR-210模拟物损害了、、、、和的表达水平，并降低了和的表达（<0.05）。总之，miR-210在猪体外胚胎发育中起重要作用。因此，我们建议抑制miR-210可减轻内质网应激并减少细胞凋亡，以支持体外胚胎生产的提高。
