[Effect of the chromatographic purification method on the isolation of prostaglandins E and F from a natural lipid mixture and their stability].

Separation of PGE2 and PGF2 alpha and their ethyl ethers on silica gel from a natural lipid mixture permits the attainment of the 97-100% purity of the substances. However, the losses for PGE2 amount to 20-25, those for PGF2 alpha to 35-40%. Thus chromatography on silica gel can be used only for analytical purposes. As regards the preparative purposes the best method of PG isolation from a natural lipid mixture is gel filtration with a preliminary separation of phospholipids from the mixture over the oleophilic sulfacationite KO-I. After separation on sephadex the preparations can be stored for up to 4-6 months at 5-8 degrees C.