Jiamboonsri Pimsumon, Sangkhun Weradesh, Wanwong Sompit
Faculty of Medicine, King Mongkut's Institute of Technology Ladkrabang, 1 Chalongkrung Road, Ladkrabang, Bangkok 10520, Thailand.
Materials Technology Program, School of Energy, Environment and Materials, King Mongkut's University of Technology Thonburi, 126 Pracha Uthit Road, Bang Mod, Bangkok 10140, Thailand.
Polymers (Basel). 2024 Dec 24;17(1):7. doi: 10.3390/polym17010007.
Methyl gallate (MG), a natural phenolic compound, exhibits in vitro synergistic activity with amoxicillin (Amox) against methicillin-resistant (MRSA), a global health concern. This study developed electrospun nanofibers incorporating MG and Amox into a poly(vinyl alcohol) (PVA)/chitosan (CS) blend to target both methicillin-susceptible (MSSA) and MRSA. The formulation was optimized, and the impact of acetic acid on antibacterial activity was evaluated using agar disc diffusion. The final formulation was fabricated and characterized using SEM, FTIR, DSC, swelling, and release behavior analyses to understand its antibacterial efficacy. Results revealed that acetic acid eliminated antibacterial activity, but MG (64 mg/mL) and Amox (2.5 mg/mL) were successfully incorporated into a PVA/CS solution prepared with deionized water. The resulting nanofiber mats featured nanoscale fibers (126 ± 45 nm) with and micron-oval beads. Despite the in vitro synergism, the MG/Amox/PVA/CS mats showed no significant improvement over MG or Amox alone against MRSA, likely due to their physicochemical properties. FTIR and DSC results confirmed molecular interactions between the active compounds and the polymer matrix, which may cause a minimal swelling and low drug release at 24 h. This study offers insights into the potential of MG/Amox-loaded nanofibers for anti-MRSA material development.
没食子酸甲酯（MG）是一种天然酚类化合物，在体外与阿莫西林（Amox）对耐甲氧西林金黄色葡萄球菌（MRSA）具有协同活性，这是一个全球关注的健康问题。本研究制备了将MG和Amox纳入聚乙烯醇（PVA）/壳聚糖（CS）共混物的电纺纳米纤维，以针对甲氧西林敏感金黄色葡萄球菌（MSSA）和MRSA。对配方进行了优化，并使用琼脂纸片扩散法评估了乙酸对抗菌活性的影响。制备了最终配方，并通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、差示扫描量热法（DSC）、溶胀和释放行为分析对其进行了表征，以了解其抗菌效果。结果表明，乙酸消除了抗菌活性，但MG（64mg/mL）和Amox（2.5mg/mL）成功地纳入了用去离子水制备的PVA/CS溶液中。所得的纳米纤维垫具有纳米级纤维（126±45nm）和微米级椭圆形珠粒。尽管在体外具有协同作用，但MG/Amox/PVA/CS垫对MRSA的抗菌效果相较于单独使用MG或Amox并无显著改善，这可能归因于它们的物理化学性质。FTIR和DSC结果证实了活性化合物与聚合物基质之间的分子相互作用，这可能导致在24小时时溶胀最小且药物释放较低。本研究为负载MG/Amox的纳米纤维在抗MRSA材料开发中的潜力提供了见解。
