Universidad Autónoma de Nuevo León, Departamento de Microbiología, Facultad de Medicina y Hospital Universitario "Dr. José Eleuterio González", Monterrey, 64460, Nuevo León, Mexico.
Universidad Autónoma de Nuevo León, Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, San Nicolas de los Garza, 66455, Nuevo León, Mexico.
Microb Pathog. 2024 Sep;194:106830. doi: 10.1016/j.micpath.2024.106830. Epub 2024 Jul 29.
Pseudomonas aeruginosa infections have become a serious threat to public health due to the increasing emergence of extensively antibiotic-resistant strains and high mortality rates. Therefore, the search for new therapeutic alternatives has become crucial. In this study, the antivirulence and antibacterial activity of methyl gallate was evaluated against six clinical isolates of extensively antibiotic-resistant P. aeruginosa. Methyl gallate exhibited minimal inhibitory concentrations of 256-384 μg/mL; moreover, the use of subinhibitory concentrations of the compound inhibited biofilm formation, swimming, swarming, proteolytic activity, and pyocyanin production. Methyl gallate plus antipseudomonal antibiotics showed a synergistic effect by reduced the MICs of ceftazidime, gentamicin and meropenem. Furthermore, the potential therapeutic effect of methyl gallate was demonstrated in an infection model. This study evidenced the antivirulence and antimicrobial activity of methyl gallate as a therapeutic alternative against P. aeruginosa.
铜绿假单胞菌感染由于广泛耐药菌株的不断出现和高死亡率，已成为严重的公共卫生威胁。因此，寻找新的治疗方法变得至关重要。在这项研究中，评价了没食子酸甲酯对 6 株临床广泛耐药铜绿假单胞菌的抗毒力和抗菌活性。没食子酸甲酯的最小抑菌浓度为 256-384μg/mL；此外，使用该化合物的亚抑菌浓度可抑制生物膜形成、泳动、群集、蛋白水解活性和绿脓菌素的产生。没食子酸甲酯与抗假单胞菌抗生素联合使用可降低头孢他啶、庆大霉素和美罗培南的 MIC，表现出协同作用。此外，在感染模型中证明了没食子酸甲酯的潜在治疗效果。本研究证明了没食子酸甲酯的抗毒力和抗菌活性，可作为治疗铜绿假单胞菌的一种替代方法。
