使用烷基化寡核苷酸衍生物对一段365个核苷酸长的DNA片段进行序列特异性化学修饰。
Sequence-specific chemical modification of a 365-nucleotide-long DNA fragment with an alkylating oligonucleotide derivative.
作者信息
Vlassov V V, Gaidamakov S A, Gorn V V, Grachev S A
出版信息
FEBS Lett. 1985 Mar 25;182(2):415-8. doi: 10.1016/0014-5793(85)80345-8.
PMID:3979561
Abstract
An aromatic 2-chloroethylamino group was attached to the 5'-terminal phosphate of the oligodeoxyribonucleotide pCCCTCTTTCTT. The oligonucleotide derivative prepared was used for modification of the 365-nucleotide-long DNA fragment. It was found that modification of the fragment proceeds in a sequence-specific way at 3 guanosine residues within the sequence complementary to the oligonucleotide reagent.
摘要
将一个芳香族2-氯乙氨基连接到寡脱氧核糖核苷酸pCCCTCTTTCTT的5'-末端磷酸基团上。所制备的寡核苷酸衍生物用于修饰365个核苷酸长的DNA片段。结果发现，该片段的修饰以序列特异性方式在与寡核苷酸试剂互补的序列中的3个鸟苷残基处进行。
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