Meng Wenshu, Gao Youhe
School of Life Sciences and Medicine, Shandong University of Technology, Zibo 255000, China.
Department of Biochemistry and Molecular Biology, Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Beijing Normal University, Beijing 100875, China.
Int J Mol Sci. 2024 Dec 24;26(1):21. doi: 10.3390/ijms26010021.
Hypertension is a multifactorial and complex disease influenced by genetic and environmental factors, and it has become one of the most serious public health challenges. This study aimed to investigate the changes in hypertension based on urinary proteome. The stroke-prone spontaneously hypertensive rats (SHRSPs) model was used to examined urinary proteome changes during the development of hypertension. Urine proteome profiling was conducted at months 1, 4, 8, 10, 12, and 14 using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Given that the progression of hypertension may vary among individuals, each rat was compared before and after hypertension developed to screen for differential proteins. Differential proteins in each rat can be enriched into some important biological processes and pathways associated with hypertension, such as the regulation of systemic arterial blood pressure by renin-angiotensin, renin-angiotensin signaling, response to glucocorticoid and glucocorticoid receptor signaling, calcium transport I, aldosterone adipocyte signaling pathway, apelin adipocyte signaling pathway, and oxidative stress response. The biological processes and pathways enriched at the same time point in the progression of hypertension differed significantly among different rat individuals. This study demonstrated that the changes in hypertension can be reflected in urine proteins. Urinary proteomics has potential in researching the mechanisms underlying hypertension, discovering new drug targets, and developing personalized strategies for antihypertensive treatment.
高血压是一种受遗传和环境因素影响的多因素复杂疾病，已成为最严峻的公共卫生挑战之一。本研究旨在基于尿蛋白质组研究高血压的变化。采用易卒中自发性高血压大鼠（SHRSPs）模型，检测高血压发展过程中尿蛋白质组的变化。在第1、4、8、10、12和14个月时，使用液相色谱-串联质谱（LC-MS/MS）进行尿蛋白质组分析。鉴于高血压的进展在个体间可能存在差异，对每只大鼠在高血压发生前后进行比较，以筛选差异蛋白。每只大鼠中的差异蛋白可富集到一些与高血压相关的重要生物学过程和通路中，如肾素-血管紧张素对全身动脉血压的调节、肾素-血管紧张素信号传导、对糖皮质激素和糖皮质激素受体信号的反应、钙转运I、醛固酮脂肪细胞信号通路、apelin脂肪细胞信号通路以及氧化应激反应。在高血压进展过程中，不同大鼠个体在同一时间点富集的生物学过程和通路存在显著差异。本研究表明，高血压的变化可在尿蛋白中得到反映。尿蛋白质组学在研究高血压发病机制、发现新的药物靶点以及制定个性化的降压治疗策略方面具有潜力。
