Yang Chenglong, Xu Xiaoping, Ali Muhammad Moaaz, He Xing, Guo Wenjie, Chen Faxing, Fang Shaozhong
Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China.
The School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571700, China.
Int J Mol Sci. 2024 Dec 27;26(1):156. doi: 10.3390/ijms26010156.
The lily is a globally popular cut flower, and managing dormancy in lily bulblets is essential for continuous, year-round production. While nitric oxide (NO) has been shown to influence seed dormancy and germination, its role in dormancy release in lilies was previously unconfirmed. In this study, we investigated the effects of NO on dormancy release in lily bulblets using SNP and c-PTIO. Results showed that SNP treatment promoted dormancy release, while c-PTIO inhibited it. Measurement of endogenous NO levels in the bulbs, along with enzyme activities of NOS-like and NR and gene expression levels of and , confirmed that NO plays a role in promoting dormancy release in lilies. To further elucidate the physiological mechanisms involved, we analyzed HO levels, antioxidant enzyme activities, endogenous hormone levels, and carbohydrate metabolism in the bulbs. Findings demonstrated that NO facilitated dormancy release by increasing HO, gibberellins (GAs), indole-3-acetic acid (IAA), zeatin riboside (ZR), reducing sugars, and by accelerating the metabolism of abscisic acid (ABA) and starch. This study provides a foundation for deeper investigation into the mechanisms underlying dormancy release in lily bulbs.
百合是一种全球广受欢迎的切花，控制百合小鳞茎的休眠对于实现全年持续生产至关重要。虽然一氧化氮（NO）已被证明会影响种子休眠和萌发，但其在百合休眠解除中的作用此前尚未得到证实。在本研究中，我们使用硝普钠（SNP）和2-（4-羧基苯基）-4,4,5,5-四甲基咪唑-1-氧基-3-氧化物（c-PTIO）研究了NO对百合小鳞茎休眠解除的影响。结果表明，SNP处理促进了休眠解除，而c-PTIO抑制了休眠解除。对鳞茎中内源性NO水平以及一氧化氮合酶样（NOS-like）和硝酸还原酶（NR）的酶活性以及 和 的基因表达水平进行测量，证实了NO在促进百合休眠解除中发挥作用。为了进一步阐明其中涉及的生理机制，我们分析了鳞茎中的过氧化氢（H₂O₂）水平、抗氧化酶活性、内源激素水平和碳水化合物代谢。研究结果表明，NO通过增加H₂O₂、赤霉素（GAs）、吲哚-3-乙酸（IAA）、玉米素核苷（ZR）、还原糖，并加速脱落酸（ABA）和淀粉的代谢来促进休眠解除。本研究为深入探究百合鳞茎休眠解除的潜在机制奠定了基础。
