Wang Shaojuan, Yi Xiaoyan, Zhang Lijuan, Ali Muhammad Moaaz, Ke Mingli, Lu Yuxian, Zheng Yiping, Cai Xuanmei, Fang Shaozhong, Wu Jian, Lin Zhimin, Chen Faxing
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Fujian Academy of Agricultural Sciences Biotechnology Institute, Fuzhou 350003, China.
Plants (Basel). 2024 May 29;13(11):1495. doi: 10.3390/plants13111495.
The Lanzhou lily ( var. ) is a variant of the Sichuan lily of the lily family and is a unique Chinese 'medicinal and food' sweet lily. Somatic cell embryogenesis of has played an important role in providing technical support for germplasm conservation, bulb propagation and improvement of genetic traits. (SERKs) are widely distributed in plants and have been shown to play multiple roles in plant life, including growth and development, somatic embryogenesis and hormone induction. Integrating the results of KEGG enrichment, GO annotation and gene expression analysis, a lily LdSERK1 gene was cloned. The full-length open reading frame of was 1875 bp, encoding 624 amino acids. The results of the phylogenetic tree analysis showed that was highly similar to rice, maize and other plant SERKs. The results of the subcellular localisation in the onion epidermis suggested that the protein was localised at the cell membrane. Secondly, we established the virus-induced gene-silencing (VIGS) system in lily scales, and the results of silencing by Tobacco rattle virus (TRV) showed that, with the down-regulation of expression, the occurrence of somatic embryogenesis and callus tissue induction in scales was significantly reduced. Finally, molecular assays from overexpression of the gene in showed that expression was significantly enhanced in the three transgenic lines compared to the wild type, and that the probability of inducing callus tissue in seed was significantly higher than that of the wild type at a concentration of 2 mg/L 2,4-D, which was manifested by an increase in the granularity of the callus tissue.
兰州百合（变种）是百合科川百合的一个变种，是中国特有的“药食同源”甜百合。兰州百合的体细胞胚胎发生在种质保存、鳞茎繁殖和遗传性状改良方面提供技术支持中发挥了重要作用。类受体蛋白激酶（SERKs）广泛分布于植物中，并已证明在植物生命中发挥多种作用，包括生长发育、体细胞胚胎发生和激素诱导。整合KEGG富集、GO注释和基因表达分析结果，克隆了一个百合LdSERK1基因。其全长开放阅读框为1875 bp，编码624个氨基酸。系统发育树分析结果表明，该基因与水稻、玉米等植物的SERKs高度相似。洋葱表皮亚细胞定位结果表明，LdSERK1蛋白定位于细胞膜。其次，我们在百合鳞片中建立了病毒诱导基因沉默（VIGS）系统，烟草脆裂病毒（TRV）对LdSERK1基因沉默的结果表明，随着LdSERK1表达的下调，鳞片中体细胞胚胎发生和愈伤组织诱导的发生率显著降低。最后，在百合中对LdSERK1基因进行过表达的分子检测表明，与野生型相比，三个转基因株系中LdSERK1表达显著增强，在2 mg/L 2,4-D浓度下，种子诱导愈伤组织的概率显著高于野生型，表现为愈伤组织颗粒度增加。
