Rehner Costache Ana Maria Gianina, Bratu Andreea Gabriela, Bîrcă Alexandra Cătălina, Niculescu Adelina-Gabriela, Holban Alina Maria, Hudiță Ariana, Bîclesanu Florentina Cornelia, Balaure Paul Cătălin, Pangică Anna Maria, Grumezescu Alexandru Mihai, Croitoru George-Alexandru
Faculty of Medicine, Titu Maiorescu University, 031593 Bucharest, Romania.
Faculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, Gh. Polizu St. 1-7, 060042 Bucharest, Romania.
Int J Mol Sci. 2024 Dec 29;26(1):194. doi: 10.3390/ijms26010194.
This study investigates the synthesis of ZnSnO@SiO@5-FU nanoparticles as an additive for bone fillers in dental maxillofacial reconstruction. ZnSnO nanoparticles were synthesized and coated with a SiO shell, followed by the incorporation of 5-Fluorouracil (5-FU), aimed at enhancing the therapeutic properties of classical fillers. Structural analysis using X-ray diffraction confirmed that ZnSnO was the single crystalline phase present, with its crystallinity preserved after both SiO coating and 5-FU incorporation. SEM characterization revealed the micro-spherical particles of ZnSnO assembled by an agglomeration of nanorods, exhibiting dimensions and morphological characteristics that were consistent after the addition of both the SiO shell and 5-FU. Fourier-transformed infrared spectroscopy provided solid proof of the successful synthesis of ZnSnO, ZnSnO@SiO, and ZnSnO@SiO@5-FU, confirming the presence of expected functional groups. The SiO layer improved nanoparticle stability in the solution, as indicated by zeta potential measurements, while adding 5-FU significantly increased biocompatibility and targeting efficiency. The existence of the SiO shell and 5-FU is also confirmed by the hydrodynamic diameter, indicating an increase in particle size after incorporating both compounds. Antibacterial assays demonstrated a selective efficacy against Gram-positive bacteria, with ZnSnO@SiO@5-FU showing the strongest inhibitory effects. Biofilm inhibition studies further confirmed the nanoparticles' effectiveness in preventing bacterial colonization. Cytotoxicity tests on the A-431 human epidermoid carcinoma cell line revealed a dose-dependent reduction in cell viability, highlighting the potential of 5-FU for targeted cancer treatment. These findings highlight the potential of ZnSnO@SiO@5-FU nanoparticles as a multifunctional additive for bone fillers, offering enhanced antimicrobial and antitumor capabilities.
本研究探究了ZnSnO@SiO@5-FU纳米颗粒的合成，该纳米颗粒作为一种添加剂用于牙颌面重建中的骨填充材料。合成了ZnSnO纳米颗粒并包覆SiO壳层，随后加入5-氟尿嘧啶（5-FU），旨在增强传统填充材料的治疗性能。使用X射线衍射进行的结构分析证实，存在的是ZnSnO单晶相，在包覆SiO和加入5-FU后其结晶度得以保留。扫描电子显微镜（SEM）表征显示，ZnSnO的微球形颗粒由纳米棒团聚而成，在添加SiO壳层和5-FU后，其尺寸和形态特征保持一致。傅里叶变换红外光谱为成功合成ZnSnO、ZnSnO@SiO和ZnSnO@SiO@5-FU提供了确凿证据，证实了预期官能团的存在。ζ电位测量表明，SiO层提高了纳米颗粒在溶液中的稳定性，而加入5-FU显著提高了生物相容性和靶向效率。流体动力学直径也证实了SiO壳层和5-FU的存在，表明加入这两种化合物后粒径增大。抗菌试验表明对革兰氏阳性菌具有选择性疗效，其中ZnSnO@SiO@5-FU显示出最强的抑制作用。生物膜抑制研究进一步证实了纳米颗粒在防止细菌定植方面的有效性。对A-431人表皮样癌细胞系的细胞毒性测试显示，细胞活力呈剂量依赖性降低，突出了5-FU在靶向癌症治疗方面的潜力。这些发现突出了ZnSnO@SiO@5-FU纳米颗粒作为骨填充材料多功能添加剂的潜力，具有增强抗菌和抗肿瘤能力。
