Ma Huiying, Liu Xin, Zhang Rui, Li Minglong, Li Qiang, Ding Xiaodong, Xiao Jialei
Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin 150030, China.
Int J Mol Sci. 2025 Jan 2;26(1):325. doi: 10.3390/ijms26010325.
Soybean () is a vital crop that is rich in high-quality protein and edible oil for human nutrition and agriculture. Saline-alkali stress, a severe environmental challenge, significantly limits soybean productivity. In this study, we found that the nodule receptor kinase GmNARK enhances soybean tolerance to alkali stress besides nodulation. could be induced by alkali stress in soybean roots. Ectopic overexpression of the gene in Arabidopsis could significantly improve plant tolerance to alkaline stress. Moreover, overexpression or silencing of the gene in soybean hairy roots also enhanced composite soybean plant tolerance to alkaline stress on plates and in soils. Additionally, overexpression of the gene upregulated expression levels of the genes that are involved in the reactive oxygen species (ROS) signaling pathways. These findings provide a critical theoretical basis for further elucidating the role of GmNARK kinase in salt-alkali resistance and lay a foundation for improving soybean productivity under salt-alkali stress.
大豆（）是一种重要作物，富含优质蛋白质和食用油，对人类营养和农业具有重要意义。盐碱胁迫作为一种严峻的环境挑战，显著限制了大豆的产量。在本研究中，我们发现根瘤受体激酶GmNARK除了在结瘤方面发挥作用外，还能增强大豆对碱胁迫的耐受性。该基因可在大豆根中被碱胁迫诱导。在拟南芥中异位过表达该基因可显著提高植物对碱性胁迫的耐受性。此外，在大豆毛状根中过表达或沉默该基因也增强了复合大豆植株在平板和土壤中对碱性胁迫的耐受性。此外，该基因的过表达上调了参与活性氧（ROS）信号通路的基因的表达水平。这些发现为进一步阐明GmNARK激酶在盐碱抗性中的作用提供了关键的理论基础，并为提高盐碱胁迫下大豆的产量奠定了基础。
