GAPDH Gene Family in : Genome-Wide Identification, Structural Analysis, and Expression Analysis Under Drought Stress.

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an enzyme widely involved in glycolysis in animal cells and in non-metabolic processes, including apoptosis and the regulation of gene expression. GAPDH is a ubiquitous protein that plays a pivotal role in plant metabolism and handling of stress responses. However, its function in plant stress resistance remains unknown. Identification and systematic analysis of the GAPDH family in () have not been performed. Bioinformatics methods were used to analyze the physicochemical characteristics, structural characteristics, phylogenetic relationships, gene structure, motif analysis, and expression of gene family members in . We identified 12 GAPDH members in . Five types of were identified: , , , , and . genes were differentially expressed in leaves, stems, and roots of 1-year-old poplar seedlings. gene transcripts showed that and were highly expressed in the leaves. In the roots, seven genes-, , , , , , and -showed significantly high expression levels. , , , and showed decreased expression under drought conditions and recovered after re-watering. These results lay the foundation for further studies on the drought stress mechanisms of