Wen Shuting, Chen Yicheng, Yang Xingzhe, Zhang Guo, Jin Lulu, Zhang Xiaoqin, Fang Yunxia, Xue Dawei
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou 311121, China.
Int J Mol Sci. 2025 Jan 2;26(1):342. doi: 10.3390/ijms26010342.
In this study, the drought-responsive gene from barley was transferred to , and overexpression lines were obtained. The phenotypic characteristics of the transgenic plants, along with physiological indicators and transcription level changes of stress-related genes, were determined under drought treatment. Under drought stress, transgenic plants overexpressing exhibited enhanced drought tolerance and longer root lengths compared to wild-type plants. Additionally, malondialdehyde and hydrogen peroxide contents were significantly lower in transgenic lines, while superoxide dismutase activity was elevated. Quantitative RT-PCR showed that the expression levels of drought and stress response genes, including , and , were significantly upregulated. Transcriptome analysis further confirmed that regulated multiple stress tolerance pathways. In summary, the overexpression of the gene enhanced drought tolerance in by regulating multiple stress response pathways. This study provides a practical basis for improving drought-resistant barley varieties and lays a foundation for subsequent research on family genes for stress resistance in barley.
在本研究中，将来自大麦的干旱响应基因转入[具体物种名称未给出]，并获得了过表达株系。在干旱处理下，测定了转基因植株的表型特征以及胁迫相关基因的生理指标和转录水平变化。在干旱胁迫下，与野生型植株相比，过表达[基因名称未给出]的转基因植株表现出增强的耐旱性和更长的根长。此外，转基因株系中丙二醛和过氧化氢含量显著降低，而过氧化物歧化酶活性升高。定量RT-PCR表明，包括[基因名称未给出]等在内的干旱和胁迫响应基因的表达水平显著上调。转录组分析进一步证实[基因名称未给出]调控多种胁迫耐受途径。总之，[基因名称未给出]基因的过表达通过调控多种胁迫响应途径增强了[具体物种名称未给出]的耐旱性。本研究为改良抗旱大麦品种提供了实践依据，并为后续大麦抗逆[基因家族名称未给出]基因的研究奠定了基础。
