Santana-Mayor Álvaro, D'Orazio Giovanni, Rodríguez-Delgado Miguel Ángel, Socas-Rodríguez Bárbara
Departamento de Química, Área de Química Analítica, Facultad de Ciencias, Universidad de La Laguna (ULL), Avenida Astrofísico Francisco Sánchez s/n, 38206 San Cristóbal de La Laguna, Tenerife, Spain.
Istituto per i Sistemi Biologici (ISB), CNR-Consiglio Nazionale delle Ricerche, Montelibretti, 00015 Rome, Italy.
Foods. 2024 Dec 25;14(1):28. doi: 10.3390/foods14010028.
In this work, two novel (-)-menthol-based hydrophobic natural eutectic solvents with vanillin and cinnamic acid were prepared and applied as extraction solvents. In this regard, 12 endocrine disruptors, including phenol, 2,4-dimethylphenol, 2,3,6-trimethylphenol, 4--butylphenol, 4--butylphenol, 4--amylphenol, 4--hexylphenol, 4--octylphenol, 4--heptylphenol, 4--octylphenol, and 4--nonylphenol and bisphenol A, were studied in a green tea drink. A temperature-controlled liquid-liquid microextraction was used as the extraction method, and nano-liquid chromatography-ultraviolet detection was used as the separation and determination system. Different parameters affecting the compatibility of the non-ionic eutectic solvents with water-polar organic solvent mixtures and chromatographic and detection systems were optimized, including injection/dilution solvent, injection mode, mobile phase composition, and step gradient. With the same purpose, two stationary phases were tested, including XBridge C and a mixed-phase Cogent C-XBridge C. Finally, the greenness and blueness of the methodology were assessed to evaluate the environmental profile and usability of the procedure.
在本研究中，制备了两种新型的基于（-）-薄荷醇的疏水性天然低共熔溶剂，分别含有香草醛和肉桂酸，并将其用作萃取溶剂。在此方面，对绿茶饮料中的12种内分泌干扰物进行了研究，包括苯酚、2,4-二甲基苯酚、2,3,6-三甲基苯酚、4-叔丁基苯酚、4-仲丁基苯酚、4-戊基苯酚、4-己基苯酚、4-庚基苯酚、4-辛基苯酚、4-壬基苯酚以及双酚A。采用温控液-液微萃取作为萃取方法，纳米液相色谱-紫外检测作为分离和测定系统。对影响非离子低共熔溶剂与水-极性有机溶剂混合物以及色谱和检测系统兼容性的不同参数进行了优化，包括进样/稀释溶剂、进样模式、流动相组成和梯度洗脱。出于同样目的，测试了两种固定相，包括XBridge C和混合相Cogent C-XBridge C。最后，对该方法的绿色度和蓝色度进行了评估，以评价该方法的环境概况和实用性。
