Wang Zhao, Li Yanqi, Xi Xiaoxue, Zou Qichao, Zhang Yuexing
Equine Science Research and Horse Doping Control Laboratory, Hubei Provincial Engineering Research Center of Racing Horse Detection and Application Transformation, Wuhan Business University, Wuhan 430056, China.
Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry & Chemical Engineering, Hubei University, Wuhan 430062, China.
Sensors (Basel). 2024 Dec 26;25(1):70. doi: 10.3390/s25010070.
In this paper, a novel molecularly imprinted polymer membrane modified glassy carbon electrode for electrochemical sensors (MIP-OH-MWCNTs-GCE) for epinephrine (EP) was successfully prepared by a gel-sol method using an optimized functional monomer oligosilsesquioxane-AlO sol-ITO composite sol (ITO-POSS-AlO). Hydroxylated multi-walled carbon nanotubes (OH-MWCNTs) were introduced during the modification of the electrodes, and the electrochemical behavior of EP on the molecularly imprinted electrochemical sensors was probed by the differential pulse velocity (DPV) method. The experimental conditions were optimized. Under the optimized conditions, the response peak current values showed a good linear relationship with the epinephrine concentration in the range of 0.0014-2.12 μM, and the detection limit was 4.656 × 10 M. The prepared molecularly imprinted electrochemical sensor was successfully applied to the detection of actual samples of horse serum with recoveries of 94.97-101.36% (RSD), which indicated that the constructed molecularly imprinted membrane electrochemical sensor has a high detection accuracy for epinephrine in horse blood, and that it has a better value for practical application.
本文采用凝胶-溶胶法，使用优化的功能单体低聚倍半硅氧烷-氧化铝溶胶-氧化铟锡复合溶胶（ITO-POSS-AlO）成功制备了一种用于肾上腺素（EP）电化学传感器的新型分子印迹聚合物膜修饰玻碳电极（MIP-OH-MWCNTs-GCE）。在电极修饰过程中引入了羟基化多壁碳纳米管（OH-MWCNTs），并采用差分脉冲伏安法（DPV）研究了EP在分子印迹电化学传感器上的电化学行为。对实验条件进行了优化。在优化条件下，响应峰电流值与肾上腺素浓度在0.0014 - 2.12 μM范围内呈现良好的线性关系，检测限为4.656×10⁻⁷ M。所制备的分子印迹电化学传感器成功应用于马血清实际样品的检测，回收率为94.97 - 101.36%（相对标准偏差），这表明构建的分子印迹膜电化学传感器对马血中肾上腺素具有较高的检测准确度，具有较好的实际应用价值。
