Academy of Scientific and Innovative Research (AcSIR), CSIR-Human Resource Development Centre, (CSIR-HRDC) Campus, Postal Staff College Area, Sector 19, Kamla Nehru Nagar, Ghaziabad, Uttar Pradesh, 201002, India.
CSIR-National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi, 110012, India.
Mikrochim Acta. 2024 Nov 5;191(12):727. doi: 10.1007/s00604-024-06781-8.
An electrochemical sensor was developed for the sensitive and selective detection of sulfadiazine (SDZ), based on a molecularly imprinted polymer (MIP) film formed on an indium tin oxide (ITO) electrode through a self-assembly process. The SDZ-imprinted ITO electrode (SDZ-MIP/APTES-ITO) was prepared through in situ polymerization using sulfadiazine, methacrylic acid (MAA), ethylene glycol dimethacrylate (EGDMA), and 2,2'-azobisisobutyronitrile (AIBN) as the template, functional monomer, cross-linker, and initiator respectively. Before polymerization, the ITO electrode was functionalized with 3-aminopropyltriethoxysilane (APTES) to promote covalent attachment of the polymer to the electrode. After polymerization, the template molecule SDZ was removed to create selective recognition sites, forming the molecularly imprinted polymer electrode (MIP/APTES-ITO), which facilitates sulfadiazine detection. The sensor's performance was evaluated using cyclic and differential pulse voltammetry, demonstrating a linear response in the sulfadiazine concentration range 0.1 to 300 μM, with a detection limit of 0.11 μM. The MIP-based sensor exhibited good reproducibility, repeatability, selectivity, and stability in sulfadiazine detection. Its practical applicability was confirmed by the successful quantification of sulfadiazine in spiked milk samples.
研制了一种基于分子印迹聚合物（MIP）膜的电化学传感器，用于灵敏和选择性检测磺胺嘧啶（SDZ）。该 MIP 膜通过自组装过程形成于氧化铟锡（ITO）电极上。通过原位聚合，以磺胺嘧啶、甲基丙烯酸（MAA）、乙二醇二甲基丙烯酸酯（EGDMA）和 2,2'-偶氮二异丁腈（AIBN）分别作为模板、功能单体、交联剂和引发剂，制备了磺胺嘧啶印迹的 ITO 电极（SDZ-MIP/APTES-ITO）。在聚合之前，ITO 电极用 3-氨丙基三乙氧基硅烷（APTES）功能化，以促进聚合物与电极的共价键合。聚合后，模板分子 SDZ 被去除，形成具有选择性识别位点的分子印迹聚合物电极（MIP/APTES-ITO），从而促进磺胺嘧啶的检测。通过循环伏安法和差分脉冲伏安法评估了传感器的性能，结果表明在磺胺嘧啶浓度范围为 0.1 至 300 μM 时具有线性响应，检测限为 0.11 μM。基于 MIP 的传感器在磺胺嘧啶检测中表现出良好的重现性、重复性、选择性和稳定性。通过成功地对加标牛奶样品中的磺胺嘧啶进行定量，验证了其实际应用的可行性。
