Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, College of Chemistry and Chemical Engineering, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, Wuhan 430062, PR China.
Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, College of Chemistry and Chemical Engineering, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, Wuhan 430062, PR China.
Biosens Bioelectron. 2015 Feb 15;64:94-101. doi: 10.1016/j.bios.2014.08.053. Epub 2014 Aug 27.
In this study, we designed a novel molecularly imprinted polymer (MIP), oligomeric methyl silsesquioxane (O-MSSQ)-TiO2 composite sol, which was made using a sol-gel reaction. This polymer has structural rigidity and high surface area of O-MSSQ, as well as high bio-compatibility and relatively good conductivity of the TiO2. Next, a sensitive and selective imprinted electrochemical sensor was successfully constructed for the direct detection of procainamide hydrochloride by molecularly imprinting a film onto the surface of a glassy carbon electrode. Adding TiO2 resulted in a noticeable enhancement in the sensitivity of the MIP sensor. The performance of the O-MSSQ-TiO2 film was discussed, and the optimal conditions for detection were determined. The oxidative peak current increased linearly with the concentration of procainamide hydrochloride in the range of 4.00 × 10(-9)-4.97 × 10(-5) M using differential pulse voltammetry, and the detection limit was 1.30 × 10(-9) M with S/N = 3. Furthermore, the sensor was applied to determine the procainamide hydrochloride content in a human blood serum sample. The recoveries of the sensors varied from 96.77% to 101.35%, indicating that the prepared sensor might be promising for the determination of procainamide hydrochloride in clinical tests. Moreover, the imprinted electrochemical sensor was used to selectively detect procainamide hydrochloride. The analytical application was conducted successfully and yielded accurate and precise results.
在这项研究中，我们设计了一种新型的分子印迹聚合物（MIP），即低聚甲基倍半硅氧烷（O-MSSQ）-TiO2 复合溶胶，该溶胶是通过溶胶-凝胶反应制备的。该聚合物具有 O-MSSQ 的结构刚性和高比表面积，以及 TiO2 的高生物相容性和相对较好的导电性。接下来，通过在玻碳电极表面分子印迹薄膜，成功构建了用于直接检测盐酸普鲁卡因胺的灵敏和选择性印迹电化学传感器。添加 TiO2 可显著提高 MIP 传感器的灵敏度。讨论了 O-MSSQ-TiO2 薄膜的性能，并确定了检测的最佳条件。使用差分脉冲伏安法，在 4.00×10(-9)-4.97×10(-5) M 的范围内，O-MSSQ-TiO2 薄膜的氧化峰电流与盐酸普鲁卡因胺的浓度呈线性关系，检测限为 1.30×10(-9) M，信噪比为 3。此外，该传感器还用于测定人血清样品中盐酸普鲁卡因胺的含量。传感器的回收率在 96.77%至 101.35%之间变化，表明所制备的传感器可能有望用于临床测试中盐酸普鲁卡因胺的测定。此外，印迹电化学传感器还用于选择性检测盐酸普鲁卡因胺。该分析应用成功进行，结果准确、精密。
