Cox G N, Carr S, Kramer J M, Hirsh D
Genetics. 1985 Mar;109(3):513-28. doi: 10.1093/genetics/109.3.513.
In Caenorhabditis elegans collagens comprise a dispersed family of 40-150 genes, the majority of which probably code for collagen proteins found in the animal's cuticle. The conserved (Gly-X-Y)n triple helix coding sequence of collagen genes has facilitated the isolation of a large number of C. elegans collagen genes by recombinant DNA methods. We have begun a study of the chromosomal organization of these genes by screening laboratory strains of C. elegans for DNA polymorphisms in the regions surrounding collagen genes. Polymorphisms near seven genes have been identified and have been used as phenotypic markers in genetic crosses to assign the genes to linkage groups II, III, IV, and X. Four genes are shown by multifactor crosses to map to a 2-3 map unit interval between unc-24 and unc-22 on chromosome IV.
在秀丽隐杆线虫中，胶原蛋白由一个包含40 - 150个基因的分散家族组成，其中大多数基因可能编码动物角质层中发现的胶原蛋白。胶原蛋白基因保守的（甘氨酸 - X - 酪氨酸）n三螺旋编码序列，通过重组DNA方法促进了大量秀丽隐杆线虫胶原蛋白基因的分离。我们已经开始通过筛选秀丽隐杆线虫实验室菌株中胶原蛋白基因周围区域的DNA多态性，来研究这些基因的染色体组织。已经鉴定出七个基因附近的多态性，并将其用作遗传杂交中的表型标记，以将这些基因分配到连锁群II、III、IV和X。通过多因素杂交显示，四个基因定位于染色体IV上unc - 24和unc - 22之间2 - 3个图距单位的区间。
