Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, England.
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7821-5. doi: 10.1073/pnas.83.20.7821.
A technique for digital characterization and comparison of DNA fragments, using restriction enzymes, is described. The technique is being applied to fragments from the nematode Caenorhabditis elegans (i) to facilitate cross-indexing of clones emanating from different laboratories and (ii) to construct a physical map of the genome. Eight hundred sixty clusters of clones, from 35 to 350 kilobases long and totaling about 60% of the genome, have been characterized.
本文介绍了一种利用限制酶对 DNA 片段进行数字化特征描述和比较的技术。该技术正应用于线虫秀丽隐杆线虫的片段，以促进不同实验室来源的克隆的交叉索引，以及构建基因组的物理图谱。已经对 860 个克隆簇进行了特征描述，这些克隆簇的长度从 35 到 350 千碱基不等，总共约占基因组的 60%。
