Technical variables in vitreous fluorophotometry.

The technical variables that affect vitreous fluorophotometry are presented. Serum fluorescein pharmacokinetics are discussed with respect to early and late fluorescein appearance in the vitreous. The problem of axial resolution was analyzed systematically and parameters to minimize or eliminate choroidal fluorescence were defined. The timing of fluorophotometry and the effects of eye movement on fluorescein spread appear to be important factors. A vitreous diffusion model is presented that may minimize artifact introduced by these factors.