Ramasamy Sriramprabha, Madhu Sekar, Choi Jungil
Department of Mechanical Engineering, Ajou University, South Korea.
Department of Mechanical Engineering, Ajou University, South Korea.
Bioelectrochemistry. 2025 Jun;163:108902. doi: 10.1016/j.bioelechem.2025.108902. Epub 2025 Jan 9.
Bloodstream bacterial infections, a major health concern due to rising sepsis rates, require prompt, cost-effective diagnostics. Conventional methods, like CO-based transduction, face challenges such as volatile metabolites, delayed gas-phase signaling, and the need for additional instruments, whereas electrochemical sensors provide rapid, sensitive, and efficient real-time detection. In this study, we developed a bioreceptor-free Prussian blue (PB) sensor platform for real-time bacterial growth monitoring in blood culture. PB thin films were electrodeposited onto a screen-printed carbon electrode (SPCE) via cyclic voltammetry (CV) technique under optimal conditions. The electrochemical performance of PB/SPCE was assessed using differential pulse voltammetry (DPV) against exoelectrogenic bacteria, including E. coli, P. aeruginosa, S. aureus, and E. faecalis. The proposed sensor exhibited surface-controlled electrochemical kinetics and bacteria-driven metal reduction from PB to Prussian white (PW), facilitated by extracellular electron transfer (EET). It showed significant sensitivity with an extensive detection range of 10-10 CFU/mL for E. coli and S. aureus, and 10-10 CFU/mL for P. aeruginosa and E. faecalis, with reliable detection limits. The sensor accessed the viability of the pathogen within 3 hrs, offering a rapid, efficient alternative to traditional, labor-intensive methods for blood-based diagnostics.
血流细菌感染因败血症发病率上升而成为主要的健康问题，需要快速且经济高效的诊断方法。传统方法，如基于一氧化碳的传感技术，面临挥发性代谢物、气相信号延迟以及需要额外仪器等挑战，而电化学传感器可提供快速、灵敏且高效的实时检测。在本研究中，我们开发了一种无生物受体的普鲁士蓝（PB）传感器平台，用于监测血培养中的细菌实时生长情况。在最佳条件下，通过循环伏安法（CV）技术将PB薄膜电沉积到丝网印刷碳电极（SPCE）上。使用差分脉冲伏安法（DPV）针对包括大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌和粪肠球菌在内的产电细菌评估PB/SPCE的电化学性能。所提出的传感器表现出表面控制的电化学动力学以及细菌驱动的从PB到普鲁士白（PW）的金属还原，这一过程由细胞外电子转移（EET）促进。它对大肠杆菌和金黄色葡萄球菌显示出显著的灵敏度，检测范围为10⁻¹⁰ CFU/mL，对铜绿假单胞菌和粪肠球菌为10⁻¹⁰ CFU/mL，具有可靠的检测限。该传感器在3小时内即可确定病原体的活力，为基于血液的诊断中的传统劳动密集型方法提供了一种快速、高效的替代方案。
