Mengxuan Qi, Xinyue Tian, Yuqing Chen, Yongkang Lu, Yanping Zhang
Anhui Province Key Laboratory of Forest Resources and Silviculture, School of Forestry and Landscape Architecture, AnHui Agricultural University, HeFei 230036, PR China.
Anhui Province Key Laboratory of Forest Resources and Silviculture, School of Forestry and Landscape Architecture, AnHui Agricultural University, HeFei 230036, PR China.
Plant Sci. 2025 Mar;352:112385. doi: 10.1016/j.plantsci.2025.112385. Epub 2025 Jan 9.
Trichome development and anthocyanin accumulation are regulated by a complex regulatory network, the MBW complexe consists of MYB, bHLH, and WD40 transcription factors. In this study, two sequences, named PaTTG1.1, and PaTTG1.2, were cloned and functionally characterized from Platanus acerifolia. Quantitative real-time PCR results showed that PaTTG1 genes were expressed in the trichomes and red leaves. Overexpression of PaTTG1.1 and PaTTG1.2 genes in Arabidopsis ttg1 mutants restored the phenotypes of ttg1 mutants that were glabrous and lacked purple anthocyanins in hypocotyls and seeds. In transgenic plants, the expression levels of the trichome regulation-related genes AtCPC, AtTRY, AtETC1, AtMYB23, and AtGL2, as well as early and late biosynthetic genes related to anthocyanin biosynthesis, were significantly upregulated. The results of the yeast two-hybrid showed that PaTTG1.1 and PaTTG1.2 proteins could physically interact with both bHLH and R2R3-MYB transcription factors from Arabidopsis and P. Acerifolia. Taken together, the results presented in this study suggest that the two PaTTG1 genes share similar functions in the regulation of trichomes and anthocyanins. However, there may be some differences in their regulatory mechanisms.
表皮毛发育和花青素积累受一个复杂调控网络的调节，MBW复合物由MYB、bHLH和WD40转录因子组成。在本研究中，从二球悬铃木中克隆了两个序列，命名为PaTTG1.1和PaTTG1.2，并对其进行了功能鉴定。实时定量PCR结果表明，PaTTG1基因在表皮毛和红叶中表达。在拟南芥ttg1突变体中过表达PaTTG1.1和PaTTG1.2基因恢复了ttg1突变体无毛且下胚轴和种子中缺乏紫色花青素的表型。在转基因植物中，表皮毛调控相关基因AtCPC、AtTRY、AtETC1、AtMYB23和AtGL2以及与花青素生物合成相关的早期和晚期生物合成基因的表达水平显著上调。酵母双杂交结果表明，PaTTG1.1和PaTTG1.2蛋白可与拟南芥和二球悬铃木的bHLH和R2R3-MYB转录因子发生物理相互作用。综上所述，本研究结果表明，两个PaTTG1基因在表皮毛和花青素调控中具有相似功能。然而，它们的调控机制可能存在一些差异。
