Pang Si, Cai Xiang, Yang Lin, Zhou Jingzhou, Li Xiaodi, Xia Siqing
Eco-environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua 321004, China.
Environ Res. 2025 Mar 1;268:120827. doi: 10.1016/j.envres.2025.120827. Epub 2025 Jan 10.
The hydrogen-based partial denitrification coupled with anammox (H-PDA) biofilm system effectively achieves low-carbon and high-efficiency biological nitrogen removal. However, the effects and biological interaction mechanism of H flux with the H-PDA system have not yet been understood. This study assessed the effects of H flux on interactions among anammox bacteria (AnAOB), denitrifying bacteria (DB), and sulfate-reducing bacteria (SRB) coexisting in a H-PDA system. Results showed the simultaneous removal of 40 mg/L ammonium nitrogen (NH-N) and 50 mg/L nitrate nitrogen (NO-N) in the H-PDA system at a flux of 0.13-0.14 e eq/(m·d) without additional organic carbon. Candidatus_Brocadia was involved in H oxidation and was negatively associated with the heterotrophic Thauera genus (DB). The expression of nirS and dsrA was increased to 5.6 × 10 copies/gSS and 2.1 × 10 copies/gSS, respectively, with excessive H flux (0.17 e eq/(m·d). This study provides technical guidance for understanding and applying the H-PDA technology for low-carbon wastewater treatment.
基于氢气的部分反硝化耦合厌氧氨氧化（H-PDA）生物膜系统能够有效实现低碳高效生物脱氮。然而，氢气通量对H-PDA系统的影响及生物相互作用机制尚未明确。本研究评估了氢气通量对H-PDA系统中厌氧氨氧化菌（AnAOB）、反硝化菌（DB）和硫酸盐还原菌（SRB）之间相互作用的影响。结果表明，在无额外有机碳的情况下，H-PDA系统在通量为0.13-0.14 e eq/(m·d)时能够同时去除40 mg/L的氨氮（NH-N）和50 mg/L的硝态氮（NO-N）。“Candidatus_Brocadia”参与氢气氧化，且与异养型陶厄氏菌属（反硝化菌）呈负相关。当氢气通量过高（0.17 e eq/(m·d)）时，nirS和dsrA的表达量分别增加至5.6×10拷贝/gSS和2.1×10拷贝/gSS。本研究为理解和应用H-PDA技术处理低碳废水提供了技术指导。
