Kamei Tatsuru, Eamrat Rawintra, Shinoda Kenta, Tanaka Yasuhiro, Kazama Futaba
Interdisciplinary Research Centre for River Basin Environment, University of Yamanashi 4-3-11, Kofu, Yamanashi, Japan E-mail:
Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi 4-3-11, Takeda, Kofu, Yamanashi, Japan.
Water Sci Technol. 2019 Mar;79(5):975-984. doi: 10.2166/wst.2018.459.
Nitrate removal during anaerobic ammonium oxidation (anammox) treatment is a concern for optimization of the anammox process. This study demonstrated the applicability and long-term stability of the coupled anammox and hydrogenotrophic denitrification (CAHD) process as an alternative method for nitrate removal. Laboratory-scale fixed bed anammox reactors (FBR) supplied with H to support denitrification were operated under two types of synthetic water. The FBRs showed simultaneous NH-N and NO-N removal, indicating that the CAHD process can support NO-N removal during the anammox process. Intermittent H supply (e.g. 5 mL/min for a 1-L reactor, 14/6-min on/off cycle) helped maintain the CAHD process without deteriorating its performance under long-term operation and resulted in a nitrogen removal rate of 0.21 kg-N/m/d and ammonium, nitrate, and dissolved inorganic nitrogen removal efficiencies of 73.4%, 80.4%, and 77%, respectively. The microbial community structure related to the CAHD process was not influenced by changes in influent water quality, and included the anammox bacteria 'Candidatus Jettenia' and a Sulfuritalea hydrogenivorans-like species as the dominant bacteria even after long-term reactor operation, suggesting that these bacteria are key to the CAHD process. These results indicate that the CAHD process is a promising method for enhancing the efficiency of anammox process.
厌氧氨氧化（anammox）处理过程中的硝酸盐去除是优化anammox工艺时需要关注的问题。本研究证明了耦合厌氧氨氧化与氢自养反硝化（CAHD）工艺作为一种去除硝酸盐的替代方法的适用性和长期稳定性。配备氢气以支持反硝化的实验室规模固定床厌氧氨氧化反应器（FBR）在两种合成水条件下运行。FBR显示出同时去除NH-N和NO-N，这表明CAHD工艺可以在anammox过程中支持NO-N的去除。间歇性氢气供应（例如，对于1-L反应器为5 mL/min，14/6分钟开/关循环）有助于维持CAHD工艺，且在长期运行下不会使其性能恶化，氮去除率为0.21 kg-N/m/d，铵、硝酸盐和溶解无机氮的去除效率分别为73.4%、80.4%和77%。与CAHD工艺相关的微生物群落结构不受进水水质变化的影响，即使在反应器长期运行后，厌氧氨氧化细菌“Candidatus Jettenia”和一种类似嗜氢硫杆菌属的物种仍作为优势细菌，这表明这些细菌是CAHD工艺的关键。这些结果表明，CAHD工艺是提高anammox工艺效率的一种有前景的方法。
