Takeda Paula Yumi, Paula Carolina Tavares, Dias Maria Eduarda Simões, Borges André do Vale, Damianovic Márcia Helena Rissato Zamariolli
Biological Processes Laboratory (LPB), São Carlos School of Engineering (EESC), University of São Paulo (USP), Av. João Dagnone, 1100, Santa Angelina, São Carlos, São Paulo, 13563-120, Brazil.
Biological Processes Laboratory (LPB), São Carlos School of Engineering (EESC), University of São Paulo (USP), Av. João Dagnone, 1100, Santa Angelina, São Carlos, São Paulo, 13563-120, Brazil.
Chemosphere. 2025 Mar;372:144105. doi: 10.1016/j.chemosphere.2025.144105. Epub 2025 Jan 17.
Simultaneous partial nitrification, anammox, and denitrification (SNAD) process offers a promising method for the effective removal of carbon and nitrogen from wastewater. However, ensuring stability is a challenge. This study investigated operational parameters such as hydraulic retention time (HRT) and biomass retention to stabilize SNAD operation, transitioning from synthetic to anaerobically pre-treated municipal wastewater (APMW) in an upflow hybrid biofilm-granular reactor (UHR). The incorporation of hybrid biomass in the form of biofilms and granules resulted in a significant improvement in ammonium oxidation, increasing the efficiency from 45% to 60%. This outcome underscores the significance of biomass retention as a crucial parameter in achieving optimal performance. Furthermore, extending the HRT resulted in a significant improved nitrogen removal, increasing it from 40% (8h) to 70% (12h), which was attributed to the enhanced specific activities of ammonium-oxidizing bacteria (AOB) and anammox bacteria (AnAOB). Microbial characterization unveiled the emergence of partial denitrifiers (Thauera genus) and the suppression of nitrite-oxidizing bacteria (NOB) (Nitrospira genus) at low aeration rates (0.35 L min.L; estimated 0.5 mgDO.L). Notably, stable operation persisted throughout the experimental period, primarily due to the consistent nitrite supply from partial nitrification/denitrification. Our findings highlight the potential of innovative hybrid reactor configuration, for achieving stable and efficient SNAD performance in mainstream wastewater treatment.
同步短程硝化、厌氧氨氧化和反硝化（SNAD）工艺为有效去除废水中的碳和氮提供了一种很有前景的方法。然而，确保其稳定性是一项挑战。本研究调查了水力停留时间（HRT）和生物量保留等运行参数，以稳定SNAD运行，在上流式混合生物膜-颗粒反应器（UHR）中从合成废水过渡到厌氧预处理城市污水（APMW）。以生物膜和颗粒形式存在的混合生物量的加入显著提高了氨氧化效率，从45%提高到了60%。这一结果强调了生物量保留作为实现最佳性能的关键参数的重要性。此外，延长HRT导致氮去除率显著提高，从40%（8小时）提高到70%（12小时），这归因于氨氧化细菌（AOB）和厌氧氨氧化细菌（AnAOB）比活性的增强。微生物特征分析揭示了在低曝气速率（0.35 L min.L；估计0.5 mgDO.L）下部分反硝化菌（陶厄氏菌属）的出现和亚硝酸盐氧化细菌（NOB）（硝化螺菌属）的抑制。值得注意的是，在整个实验期间运行保持稳定，这主要归功于短程硝化/反硝化提供的稳定亚硝酸盐供应。我们的研究结果突出了创新混合反应器配置在主流污水处理中实现稳定高效SNAD性能的潜力。
