在水介质中使用有机配体作为荧光开启探针进行铅离子的痕量检测。

Trace level detection of Pb ion using organic ligand as fluorescent-on probes in aqueous media.

作者信息

Sadia Maria, Khan Jehangir, Khan Rizwan, Ali Shah Syed Wadood, Zada Adil, Zahoor Muhammad, Ullah Riaz, Ali Essam A

机构信息

Department of Chemistry, University of Malakand, Chakdara, Lower Dir, Khyber Pakhtunkhwa, Pakistan.

Department of Electrical Engineering, Kwangwoon University, Seoul, South Korea.

出版信息

Heliyon. 2024 Dec 12;11(1):e41125. doi: 10.1016/j.heliyon.2024.e41125. eCollection 2025 Jan 15.

In this study, an optical sensor, JA/(2,6-di((E)-benzylidene)cyclohexan-1-one), was synthesized and characterized using H NMR and FT-IR spectroscopy. The sensor exhibited high efficiency and selectivity in detecting Pb ions, even in the presence of potential interfering ions such as Mn, Cu, Co, Cr, Ni, Ce, Hg, and Cd in aqueous solutions. The interaction of JA with Pb resulted in a significant enhancement of fluorescence intensity, suggesting the formation of a stable complex. A 2:1 binding ratio between JA and Pb was confirmed through fluorometric analysis, absorption spectra, and theoretical calculations (DFT). The association constant for the complex was calculated to be 3 × 10 M. The sensor demonstrated high sensitivity towards Pb with a detection limit of 5 × 10 M. Additionally, JA was successfully reused by applying EDTA to release Pb from the sensor. Real sample analysis under optimized conditions of pH, time, and concentration of JA and Pb further validated the practical applicability of the sensor.

在本研究中，合成了一种光学传感器JA/（2,6 - 二（（E） - 亚苄基）环己烷 - 1 - 酮），并使用核磁共振氢谱（H NMR）和傅里叶变换红外光谱（FT - IR）对其进行了表征。该传感器在检测铅离子时表现出高效性和选择性，即使在水溶液中存在潜在干扰离子，如锰、铜、钴、铬、镍、铈、汞和镉的情况下也是如此。JA与铅的相互作用导致荧光强度显著增强，表明形成了稳定的络合物。通过荧光分析、吸收光谱和理论计算（密度泛函理论，DFT）证实了JA与铅的结合比为2:1。该络合物的缔合常数经计算为3×10⁶ M⁻¹。该传感器对铅表现出高灵敏度，检测限为5×10⁻⁸ M。此外，通过应用乙二胺四乙酸（EDTA）从传感器中释放铅，JA得以成功重复使用。在pH值、时间以及JA和铅浓度的优化条件下进行实际样品分析，进一步验证了该传感器的实际适用性。