Kimler B F, Park C H, Yakar D, Mies R M
Int J Radiat Oncol Biol Phys. 1985 Apr;11(4):809-16. doi: 10.1016/0360-3016(85)90315-3.
The effect of ionizing radiation on the survival of bone marrow cells from patients with acute nonlymphocytic leukemia or from hematologically normal controls was studied using colony formation as an endpoint. A modified agar culture method which incorporated daily feeding with new medium was used to allow the growth of leukemic cell colonies. Analysis of radiation-dose survival curves revealed that normal bone marrow cell populations exhibited a relatively steep slope, with values of D0 ranging from 0.5-1.3 Gy (mean = 0.82 +/- 0.22 Gy). There was essentially no shoulder to the survival curves, with Dq values ranging from less than 0 to 0.3 Gy. The leukemic cells tested displayed survival curves that did not differ qualitatively from those obtained with normal cells, i.e., steep slopes and neglible shoulders. However, the average value of the D0 (0.62 +/- 0.15 Gy) was statistically different (p less than 0.025) than that obtained for the normal cells. The results of these studies may have implications both for the use of radiation therapy for the treatment of malignant hemopoietic diseases, and for total body irradiation prior to bone marrow transplantation.
以集落形成作为终点，研究了电离辐射对急性非淋巴细胞白血病患者或血液学正常对照者骨髓细胞存活的影响。采用改良的琼脂培养方法，每天添加新鲜培养基，以促进白血病细胞集落的生长。对辐射剂量存活曲线的分析表明，正常骨髓细胞群体呈现相对陡峭的斜率，D0值范围为0.5 - 1.3 Gy（平均值 = 0.82 ± 0.22 Gy）。存活曲线基本没有坪区，Dq值范围小于0至0.3 Gy。所检测的白血病细胞显示的存活曲线与正常细胞的存活曲线在性质上没有差异，即斜率陡峭且坪区可忽略不计。然而，D0的平均值（0.62 ± 0.15 Gy）与正常细胞相比有统计学差异（p < 0.025）。这些研究结果可能对恶性造血疾病的放射治疗以及骨髓移植前的全身照射都有影响。
