Kimler B F, Park C H, Yakar D, Mies R M
Br J Cancer Suppl. 1984;6:221-5.
The effect of ionizing radiation on the growth of bone marrow cells from haematologically normal controls or from patients with acute nonlymphocytic leukaemia was studied using colony formation as an endpoint. A modified agar culture method which incorporated daily feeding with new medium was utilized to allow the growth of leukaemic cell colonies. Analysis of radiation-dose survival curves revealed that normal bone marrow cell populations exhibited a relatively steep slope, with values of D0 averaging 0.88 Gy, and essentially no shoulder to the survival curves. The leukaemic cells tested also displayed survival curves with negligible shoulders and steep slopes (average value of D0 = 0.66 Gy). The average values of D0 for normal and leukaemic cells were significantly different (P less than 0.001). The ability of hematopoietic progenitor cells to recover from the accumulation of sublethal damage was also investigated by allowing a fractionation interval between doses. As expected from the lack of significant shoulder on the survival curve, there was no evidence of recovery from sublethal damage. These results provide a rationale for the use of fractionated radiation therapy for whole-body irradiation prior to bone marrow transplant.
以集落形成作为终点，研究了电离辐射对血液学正常对照者或急性非淋巴细胞白血病患者骨髓细胞生长的影响。采用一种改良的琼脂培养方法，该方法包括每天用新鲜培养基喂养，以促进白血病细胞集落的生长。对辐射剂量存活曲线的分析表明，正常骨髓细胞群体呈现出相对陡峭的斜率，D0值平均为0.88 Gy，存活曲线基本没有坪区。所测试的白血病细胞也显示出存活曲线，坪区可忽略不计且斜率陡峭（D0平均值 = 0.66 Gy）。正常细胞和白血病细胞的D0平均值有显著差异（P小于0.001）。通过设定剂量之间的分次间隔，还研究了造血祖细胞从亚致死损伤积累中恢复的能力。正如从存活曲线缺乏明显坪区所预期的那样，没有证据表明存在从亚致死损伤中恢复的情况。这些结果为在骨髓移植前对全身照射采用分次放射治疗提供了理论依据。
