Hilderink Sarah, van der Velden Jolanda, Kuster Diederik W D
Amsterdam UMC location Vrije Universiteit Amsterdam, Physiology, De Boelelaan 1118, Amsterdam, the Netherlands.
Amsterdam Cardiovascular Sciences, Heart Failure & Arrhythmias, Amsterdam, the Netherlands.
J Mol Cell Cardiol Plus. 2024 Mar 16;8:100071. doi: 10.1016/j.jmccpl.2024.100071. eCollection 2024 Jun.
Few immortalized cardiac microvascular endothelial cell (CMEC) lines are available, particularly mouse lines. We purchased the CLU510 mCMEC line (Cedarlane), isolated by fluorescence-activated cell sorting for CD31 and VE-cadherin. The cell line has been used in previous studies, although none report CD31 or VE-cadherin expression. We analyzed endothelial profile of two vials of passage 38 cells. CD31 and VE-cadherin mRNA were hardly expressed in mCMECs compared to primary mouse lung ECs. CD31 and VE-cadherin protein levels were also negligible compared to multiple EC lines. Thus, CLU510 mCMECs beyond P38 do not harbor an endothelial phenotype. Caution should be warranted when using commercial cells and journals should carefully consider the validity of results when essential characterization of cell lines is omitted.
可供使用的永生化心脏微血管内皮细胞（CMEC）系很少，尤其是小鼠细胞系。我们购买了通过针对CD31和VE-钙黏蛋白的荧光激活细胞分选分离得到的CLU510小鼠CMEC系（Cedarlane公司）。该细胞系已在先前的研究中使用，尽管没有研究报告其CD31或VE-钙黏蛋白的表达情况。我们分析了两管传代38次的细胞的内皮细胞特征。与原代小鼠肺内皮细胞相比，mCMEC中CD31和VE-钙黏蛋白的mRNA几乎不表达。与多个内皮细胞系相比，CD31和VE-钙黏蛋白的蛋白水平也可忽略不计。因此，传代38代以后的CLU510 mCMEC不具有内皮细胞表型。使用商业细胞时应谨慎，当细胞系的基本特征未被描述时，期刊应仔细考虑研究结果的有效性。
