Fricker C R
J Appl Bacteriol. 1985 Jan;58(1):57-62. doi: 10.1111/j.1365-2672.1985.tb01429.x.
The effect of different storage procedures on the ability of Preston medium to recover campylobacters was investigated. Freshly poured media was shown to recover more campylobacters than media stored under aerobic or anaerobic conditions at room temperature or at 4 degrees C. The growth of Campylobacter laridis was greatly reduced by storage of media and although most strains of C. jejuni and C. coli were not markedly affected, the growth of one strain of C. jejuni was considerably reduced. It is recommended that freshly prepared media be used whenever possible, but if storage is necessary, then plates should be held at 4 degrees C, preferably under anaerobic conditions. These precautions may not be necessary for workers interested solely in C. jejuni or C. coli, but are essential for the optimum isolation of C. laridis.
研究了不同储存程序对普雷斯顿培养基恢复弯曲杆菌能力的影响。结果表明,新鲜倾注的培养基比在室温或4摄氏度有氧或厌氧条件下储存的培养基能恢复更多的弯曲杆菌。培养基储存会使拉氏弯曲杆菌的生长大幅减少,尽管大多数空肠弯曲杆菌和结肠弯曲杆菌菌株未受到明显影响,但一株空肠弯曲杆菌的生长显著降低。建议尽可能使用新鲜制备的培养基,但如果有必要储存,则平板应保存在4摄氏度,最好是在厌氧条件下。对于仅关注空肠弯曲杆菌或结肠弯曲杆菌的工作人员来说,这些预防措施可能不必要,但对于拉氏弯曲杆菌的最佳分离至关重要。