用于鉴定嗜热弯曲菌属的DNA探针培养确认试验。
DNA probe culture confirmation assay for identification of thermophilic Campylobacter species.
作者信息
Tenover F C, Carlson L, Barbagallo S, Nachamkin I
机构信息
Seattle Veterans Affairs Medical Center, Washington 98108.
出版信息
J Clin Microbiol. 1990 Jun;28(6):1284-7. doi: 10.1128/jcm.28.6.1284-1287.1990.
Abstract
We studied the ability of a new DNA probe-based assay system to correctly identify isolates of the thermophilic campylobacters Campylobacter jejuni, C. coli, and C. laridis grown in vitro. We examined 424 organisms, including 214 Campylobacter isolates and 210 other aerobic and anaerobic isolates. The probe assay, which uses a new homogeneous system in which all reactions take place within a single tube, demonstrated 100% accuracy, producing neither false-positive nor false-negative results. The assay does not, however, distinguish among C. jejuni, C. coli, and C. laridis.
摘要
我们研究了一种基于新型DNA探针的检测系统正确识别体外培养的嗜热弯曲菌空肠弯曲菌、大肠弯曲菌和拉氏弯曲菌分离株的能力。我们检测了424株微生物，包括214株弯曲菌分离株和210株其他需氧和厌氧分离株。该探针检测使用了一种新型均相系统，所有反应都在单个试管内进行，显示出100%的准确性，既没有产生假阳性结果，也没有产生假阴性结果。然而，该检测方法无法区分空肠弯曲菌、大肠弯曲菌和拉氏弯曲菌。
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