Kettenburg Gwenddolen, Ranaivoson Hafaliana C, Andrianianina Angelo, Andry Santino, Henry Amy R, Davis Rachel L, Laboune Farida, Longtine Elizabeth R, Godbole Sucheta, Horigan Sophia, Ruhs Emily Cornelius, Raharinosy Vololoniaina, Randriambolamanantsoa Tsiry Hasina, Lacoste Vincent, Heraud Jean-Michel, Dussart Philippe, Douek Daniel C, Brook Cara E
bioRxiv. 2025 Jan 2:2024.12.31.630946. doi: 10.1101/2024.12.31.630946.
Bats are reservoir hosts for numerous well-known zoonotic viruses, but their broader virus-hosting capacities remain understudied. are an order of enteric viruses known to cause disease across a wide range of mammalian hosts, including Hepatitis A in humans and foot-and-mouth disease in ungulates. Host-switching and recombination drive the diversification of worldwide. Divergent and (families within the ) have been described in bats across mainland Africa, but surveillance for these viruses has been rare in the Southwest Indian Ocean Islands. Bats live in close proximity to and are consumed widely as a food source by humans in Madagascar, providing opportunities for zoonotic transmission. Prior work in Madagascar has described numerous evolutionarily divergent bat viruses, some with zoonotic potential. Using metagenomic Next Generation Sequencing of urine and fecal samples obtained from three species of endemic Malagasy fruit bats ( , , and ), we recovered 13 full-length and 37 partial-length genomic sequences within the order (36 and 14 sequences), which we identify and describe here. We find evidence that genetic exchange between mainland African bat and Madagascar bat likely shaped the diversification patterns of these novel sequences through recombination events between closely related ; thus far, high host fidelity appears to have limited these viruses from spilling over into other species.
蝙蝠是众多著名人畜共患病毒的宿主，但它们更广泛的病毒承载能力仍未得到充分研究。 是一类肠道病毒，已知会在包括人类甲型肝炎和有蹄类动物口蹄疫在内的多种哺乳动物宿主中引发疾病。宿主转换和重组推动了全球范围内 的多样化。在非洲大陆的蝙蝠中已发现不同的 和 （ 科内的家族），但在印度洋西南部岛屿对这些病毒的监测却很少见。在马达加斯加，蝙蝠与人类生活距离很近，并且被广泛作为食物来源，这为人畜共患传播提供了机会。马达加斯加先前的研究描述了许多进化上不同的蝙蝠病毒，其中一些具有人畜共患潜力。通过对从三种马达加斯加特有果蝠（ 、 和 ）获取的尿液和粪便样本进行宏基因组下一代测序，我们在 目内获得了全长13个和部分长度37个基因组序列（36个 序列和14个 序列），我们在此对其进行识别和描述。我们发现证据表明，非洲大陆蝙蝠和马达加斯加蝙蝠 的基因交换可能通过密切相关的 之间的重组事件塑造了这些新序列的多样化模式；到目前为止，高宿主特异性似乎限制了这些病毒传播到其他物种。
