Toxic workplace environments, especially those involving gaslighting, are known to contribute to stress and excessive work habits, such as workaholism, which may hinder a nurse's agility-an essential skill in adapting to fast-paced healthcare environments. However, the interplay between workplace gaslighting, workaholism, and agility in nursing remains underexplored. This study aims to investigate the relationship between workplace gaslighting, workaholism, and agility among nurses, focusing on how gaslighting moderates this relationship. This study is a multicenter cross-sectional that was conducted among 594 full-time nurses from three major hospitals in Egypt. Data were collected using the Gaslighting at Work Questionnaire, Workforce Agility Scale, and Bergen Work Addiction Scale. Correlation and regression analyses were performed to test the study hypotheses. Results revealed that nurses reported moderate levels of workaholism, workplace gaslighting, and agility. Significant negative correlations were found between agility and both workplace gaslighting and workaholism. Gaslighting negatively moderated the relationship between workaholism and agility, exacerbating the impact of workaholism on nurses' ability to remain agile. The findings indicate that toxic workplace behaviors, such as gaslighting and workaholism, undermine nurses' agility. Regulatory safeguards are necessary to protect nurses from such environments, ensuring both their well-being and the quality of healthcare services. These findings call for the development of national standards and policies to address workplace mental health and ensure accountability in healthcare settings.