Jensen Per, Ozenne Brice, Meden Per, Feng Ling, Thomsen Gerda, Knudsen Lars, Steglich-Arnholm Henrik, Møller Kirsten, Thomsen Carsten, Svarer Claus, Beliveau Vincent, Mikkelsen Jens, Knudsen Gitte, H Pinborg Lars
Neurobiology Research Unit, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
Epilepsy Clinic, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
J Neuroimaging. 2025 Jan-Feb;35(1):e70002. doi: 10.1111/jon.70002.
This study aims to investigate the longitudinal changes in translocator protein (TSPO) following stroke in different brain regions and potential associations with chronic brain infarction.
Twelve patients underwent SPECT using the TSPO tracer 6-Chloro-2-(4'-123I-Iodophenyl)-3-(N,N-Diethyl)-Imidazo[1,2-a]Pyridine-3-Acetamide, as well as structural MRI, at 10, 41, and 128 days (median) after ischemic infarction in the middle cerebral artery. TSPO expression was measured in lesional (MRI lesion and SPECT lesion), connected (pons and ipsilesional thalamus), and nonconnected (ipsilesional cerebellum and contralesional occipital cortex) regions. Correlations were explored between the volume of chronic infarction and TSPO expression in nonconnected regions of interest (ROIs) at 128 days RESULTS: Throughout the study period, TSPO levels decreased by 24%-33% in lesional ROIs, while levels increased in connected ROIs by 35%-69% and in nonconnected ROIs by 53%-77%. At 128 days poststroke, TSPO expression in ipsilesional cerebellum positively correlated with chronic infarction volume (p = 0.002, r = 0.72).
This study expands the current knowledge of spatial and temporal TSPO expression in humans by quantifying TSPO changes in lesional, connected, and nonconnected brain regions at three time points after cerebral infarction as well as correlating late-stage TSPO upregulation and chronic infarction volume.
本研究旨在调查不同脑区中风后转位蛋白（TSPO）的纵向变化以及与慢性脑梗死的潜在关联。
12例患者在大脑中动脉缺血性梗死10天、41天和128天（中位数）后，使用TSPO示踪剂6-氯-2-（4'-123I-碘苯基）-3-（N，N-二乙基）-咪唑并[1,2-a]吡啶-3-乙酰胺进行单光子发射计算机断层扫描（SPECT）以及结构磁共振成像（MRI）。在病变区域（MRI病变和SPECT病变）、相连区域（脑桥和同侧丘脑）和不相连区域（同侧小脑和对侧枕叶皮质）测量TSPO表达。在128天时，探讨慢性梗死体积与不相连感兴趣区域（ROI）中TSPO表达之间的相关性。结果：在整个研究期间，病变ROI中的TSPO水平下降了24%-33%，而相连ROI中的水平上升了35%-69%，不相连ROI中的水平上升了53%-77%。在中风后128天，同侧小脑中的TSPO表达与慢性梗死体积呈正相关（p = 0.002，r = 0.72）。
本研究通过量化脑梗死三个时间点病变、相连和不相连脑区的TSPO变化以及关联晚期TSPO上调与慢性梗死体积，扩展了目前关于人类TSPO表达时空变化的知识。
