Mills R S
J Assoc Off Anal Chem. 1985 Jan-Feb;68(1):56-8.
The determination of the vitamin A concentration in fortified milk was compared using Carr-Price analysis and liquid chromatography (LC). Carr-Price analysis required saponification of the sample with alcoholic potassium hydroxide, extraction with ether, and colorimetry with antimony trichloride in chloroform. LC analysis required hexane extraction of a 71% alcohol-sample solution and centrifugation at 2000 rpm. A 100 microL aliquot of the extract was analyzed on a LiChrosorb Si-60, 5 micron column, using an ethyl ether-hexane (2 + 98) mobile phase and detection at 313 nm. Each method was statistically evaluated for precision and sample-to-sample reproducibility. The LC extraction procedure was examined for efficiency. Each LC value was divided by the Carr-Price value obtained for the same sample; an average value of 0.975 with a coefficient of variation of 6.90% was obtained. It was concluded that the procedures were statistically equivalent.
采用卡尔-普赖斯分析法和液相色谱法（LC）对强化牛奶中维生素A浓度的测定进行了比较。卡尔-普赖斯分析法需要用氢氧化钾乙醇溶液对样品进行皂化，用乙醚萃取，并用三氯化锑的氯仿溶液进行比色测定。液相色谱分析需要用己烷萃取71%的乙醇-样品溶液，并以2000转/分钟的速度离心。取100微升萃取液等分试样，在LiChrosorb Si - 60、5微米柱上进行分析，使用乙醚-己烷（2 + 98）流动相，并在313纳米处进行检测。对每种方法的精密度和样品间再现性进行了统计学评估。对液相色谱萃取程序的效率进行了考察。将每个液相色谱值除以同一样品获得的卡尔-普赖斯值；得到的平均值为0.975，变异系数为6.90%。得出的结论是，这些程序在统计学上是等效的。
