Liu Min, Zhu Chuanlin, Dong Zihe, Wang Zhangmin, Yang Huan, Li Jie, Li Ke, Shen Bo, Li Xinmin, Leng Ping, Ding Shijia, Guo Jinlin, Zhang Juan
College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
Department of Laboratory Medicine, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, 400021, China.
Mikrochim Acta. 2025 Jan 13;192(2):71. doi: 10.1007/s00604-024-06935-8.
An innovative colorimetric sensing strategy was developed for the detection of glucose by the integration of glucose aptamer, glucose oxidase (GOx), and horseradish peroxidase (HRP), termed aptamer proximal enzyme cascade reactions (APECR). In the presence of glucose, aptamer binding enables GOx to catalyze glucose oxidation into HO efficiently. Subsequently, the adjacent HRP catalyzes the oxidation of the peroxidase substrate, 2,2'-biazobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), utilizing the generated HO, resulting in a distinct color change. In comparison to the free enzymes and the HRP-GOx system, APECR exhibited higher colorimetric signal. This approach achieved glucose detection within three minutes, which was significantly faster than previous methods. This method showed good sensitivity and selectivity with a limit of detection of 0.013 mM. Moreover, the practical utility of this strategy was verified by achieving rapid detection of glucose in clinical serum samples. Hence, the developed strategy has the advantages of simple operation and rapid analysis time for the detection of glucose in human serum.
通过整合葡萄糖适体、葡萄糖氧化酶（GOx）和辣根过氧化物酶（HRP），开发了一种用于检测葡萄糖的创新比色传感策略，称为适体近端酶级联反应（APECR）。在葡萄糖存在的情况下，适体结合使GOx能够有效地将葡萄糖氧化为H₂O₂。随后，相邻的HRP利用生成的H₂O₂催化过氧化物酶底物2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）的氧化，导致明显的颜色变化。与游离酶和HRP-GOx系统相比，APECR表现出更高的比色信号。该方法在三分钟内实现了葡萄糖检测，比以前的方法快得多。该方法具有良好的灵敏度和选择性，检测限为0.013 mM。此外，通过在临床血清样本中实现葡萄糖的快速检测，验证了该策略的实际应用价值。因此，所开发的策略在检测人血清中的葡萄糖方面具有操作简单和分析时间短的优点。
