用于体外培养和功能测定的小鼠肝脏窦状内皮细胞分离和纯化方案。
Protocol for isolating and purifying murine liver sinusoidal endothelial cells for in vitro culture and functional assays.
作者信息
Papaioannou Sophia, See Jia-Xiang, Baljkas Tinja, Reiners-Koch Philipp, Winkler Manuel, Cerwenka Adelheid, Stojanovic Ana
机构信息
Department of Immunobiochemistry, Mannheim Institute for Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany; European Center for Angioscience (ECAS), Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, Heidelberg University, and Center of Excellence in Dermatology, Mannheim, Germany.
出版信息
STAR Protoc. 2025 Mar 21;6(1):103554. doi: 10.1016/j.xpro.2024.103554. Epub 2025 Jan 12.
Liver sinusoidal endothelial cells (LSECs) line the liver sinusoids and play a crucial role in liver function. Isolating LSECs is beneficial for their functional evaluation in vitro. Here, we provide a protocol for obtaining purified LSECs from mice via gradient centrifugation and magnetic cell sorting (MACS), yielding cells suitable for culture and downstream analyses. We describe steps for culturing the purified LSECs and demonstrate their evaluation by flow cytometry. For complete details on the use and execution of this protocol, please refer to Papaioannou et al..
肝窦内皮细胞（LSECs）排列在肝血窦内，对肝功能起着至关重要的作用。分离LSECs有利于其体外功能评估。在此，我们提供了一种通过梯度离心和磁珠细胞分选（MACS）从小鼠获得纯化LSECs的方案，所获细胞适用于培养和下游分析。我们描述了纯化LSECs的培养步骤，并通过流式细胞术展示了对其的评估。有关本方案使用和实施的完整详细信息，请参考帕帕约安努等人的研究。
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