从鼠组织中分离内皮细胞的方案：小肠、结肠、心脏和肝脏。

Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, & Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, Leuven, Belgium.

Translational Cancer Research Unit, GZA Hospitals Sint-Augustinus, Antwerp 2610, Belgium and Center for Oncological Research, University of Antwerp, Antwerp 2000, Belgium.

STAR Protoc. 2021 May 1;2(2):100489. doi: 10.1016/j.xpro.2021.100489. eCollection 2021 Jun 18.

Endothelial cells (ECs) from the small intestine, colon, liver, and heart have distinct phenotypes and functional adaptations that are dependent on their physiological environment. Gut ECs adapt to low oxygen, heart ECs to contractile forces, and liver ECs to low flow rates. Isolating high-purity ECs in sufficient quantities is crucial to study their functions. Here, we describe protocols combining magnetic and fluorescent activated cell sorting for rapid and reproducible EC purification from four adult murine tissues. For complete details on the use and execution of these protocols, please refer to Kalucka et al. (2020).

肠、结肠、肝和心脏的内皮细胞（ECs）具有不同的表型和功能适应性，这取决于它们的生理环境。肠道 ECs 适应低氧环境，心脏 ECs 适应收缩力，而肝脏 ECs 适应低流速。分离足够数量的高纯度 ECs 对于研究其功能至关重要。在这里，我们描述了结合使用磁性和荧光激活细胞分选技术从四种成年鼠组织中快速、可重复地纯化 EC 的方案。有关这些方案的使用和执行的完整详细信息，请参阅 Kalucka 等人。（2020 年）。