采用超高效液相色谱-四极杆-静电场轨道阱质谱联用技术探究石斛属植物中抗动脉粥样硬化潜在生物活性成分的异同。

Exploring similarities and differences in anti-atherosclerotic potential bioactives among Dendrobium species by UPLC-Q-Exactive Orbitrap MS.

作者信息

Zhang Yu, Chen Xi, Tang Oushan, Cheng Keyun, Ge Lijun, Lu Weibo, Zheng Jing, Wu Yeshun, Wang Si-Wei, Zeng Xi-Xi, Xue Jing, Cui Yiwei, Ji Liting, Shen Qing

机构信息

Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China.

School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.

出版信息

NPJ Sci Food. 2025 Jan 13;9(1):6. doi: 10.1038/s41538-025-00371-5.

Atherosclerosis is a primary cause of cardiovascular disease, straining healthcare systems. Dendrobium officinale, a widely used food-medicine homology, has demonstrated anti-atherosclerotic (anti-AS) properties, with other species listed in pharmacopoeias exhibiting similar effects. However, their efficacy varies, and the impact of interspecies variations on compounds and mechanisms in Dendrobium's anti-AS effects remains unclear. This study aimed to explore the anti-AS compounds and mechanisms across various Dendrobium species. The chemical composition of D. fimbriatum, D. officinale, D. devonianum, D. gratiosissimum, and D. catenatum was analyzed using UPLC-Q-Exactive Orbitrap MS. Network pharmacology predicted the pharmacological basis and molecular mechanisms. Molecular docking experiments assessed the binding affinity of the identified compounds with target proteins. A total of 12 different and 65 common components were identified. Key therapeutic targets included SRC, STAT3, and PIK3CA, along with relevant signaling pathways linked to AS prevention. The study provides insights into interspecies differences in Dendrobium's anti-AS properties.

动脉粥样硬化是心血管疾病的主要病因，给医疗系统带来了压力。铁皮石斛是一种广泛使用的药食同源植物，已显示出抗动脉粥样硬化（抗AS）特性，药典中列出的其他石斛品种也表现出类似效果。然而，它们的功效各不相同，种间差异对石斛抗AS作用中化合物和机制的影响仍不清楚。本研究旨在探索不同石斛品种的抗AS化合物和机制。采用超高效液相色谱-四极杆-静电场轨道阱质谱联用仪（UPLC-Q-Exactive Orbitrap MS）分析了流苏石斛、铁皮石斛、滇桂石斛、鼓槌石斛和链珠石斛的化学成分。网络药理学预测了其药理基础和分子机制。分子对接实验评估了所鉴定化合物与靶蛋白的结合亲和力。共鉴定出12种不同成分和65种共同成分。关键治疗靶点包括SRC、STAT3和PIK3CA，以及与预防AS相关的信号通路。该研究为石斛抗AS特性的种间差异提供了见解。